Use of a highly sensitive quantitative telomerase assay in intracytoplasmic sperm injection programmes for the treatment of 47,XXY non-mosaic Klinefelter men

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dc.contributor.authorYamamoto, Y.en
dc.contributor.authorSofikitis, N.en
dc.contributor.authorKaponis, A.en
dc.contributor.authorGeorgiou, J.en
dc.contributor.authorGiannakis, D.en
dc.contributor.authorMamoulakis, Chen
dc.contributor.authorLoutradis, D.en
dc.contributor.authorYiannakopoulos, X.en
dc.contributor.authorMio, Y.en
dc.contributor.authorMiyagawa, I.en
dc.contributor.authorChatzikyziakidou, A.en
dc.date.accessioned2015-11-24T18:55:44Z
dc.date.available2015-11-24T18:55:44Z
dc.identifier.issn0303-4569-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18920
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectBiopsyen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectIn Situ Hybridization, Fluorescenceen
dc.subjectKaryotypingen
dc.subjectKlinefelter Syndrome/*genetics/therapyen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectMosaicismen
dc.subjectSensitivity and Specificityen
dc.subject*Sperm Injections, Intracytoplasmicen
dc.subjectTelomerase/*metabolismen
dc.subjectTestis/pathologyen
dc.titleUse of a highly sensitive quantitative telomerase assay in intracytoplasmic sperm injection programmes for the treatment of 47,XXY non-mosaic Klinefelter menen
heal.abstractWe evaluated the role of the sensitive quantitative telomerase assay (SQTA) in the management of men with non-mosaic Klinefelter's syndrome (KS). Diagnostic testicular biopsy (DTB) was performed in 24 men with KS. A part of the DTB was stained and the remaining fragment was processed for the SQTA. After 3-18 months, a therapeutic testicular biopsy (TTB) was performed in the same testicle and the recovered specimens were processed to identify spermatozoa. Men with a SQTA outcome equal to 0.00 Units microg-1 protein (n = 7) demonstrated therapeutic testicular biopsy material that was negative for spermatogenic cells. In five men with a SQTA outcome of 8.11-38.03 Units microg-1, the most advanced germ cell was the spermatogonium/primary spermatocyte. In the remaining 12 men, the most advanced spermatogenic cell in the TTB was the spermatozoon. In these men, the SQTA outcome was equal to 25.76-92.68 Units microg-1 protein. Using 39.00 Units microg-1 protein as a cut-off value, the accuracy of the SQTA in identifying men positive for spermatozoa was 91.6%. It appears that the SQTA has a role for identifying non-mosaic KS men who have testicular spermatozoa.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/12220229-
heal.journalNameAndrologiaen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2002-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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