Glucose oxidase-produced H2O2 induces Ca2+-dependent DNA damage in human peripheral blood lymphocytes
Φόρτωση...
Ημερομηνία
Συγγραφείς
Panayiotidis, M.
Tsolas, O.
Galaris, D.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Free Radic Biol Med
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
DNA of lymphocytes from human peripheral blood was analyzed by using the single cell gel electrophoresis technique (comet assay). The cells were used either as received from the donors or after treatment with various concentrations of the H2O2-generating enzyme glucose oxidase, in order to achieve a continuous flow of H2O2. The formation of single strand breaks (SSB) was dose-related but the time course of the induction of SSB by relatively low concentrations of glucose oxidase was of a biphasic mode with a fast increase 2 to 5 min after the addition of glucose oxidase followed by a gradual decrease toward the original base level during the next 35 to 60 min. This response of the cells appears to be based on the activation of already existing defense system(s) because it was shown that H2O2 is continuously released during the reaction time and the inhibition of protein synthesis does not affect the observed pattern. Supplementation of the growth medium with various antioxidants resulted in substantial protection only when the agents were taken up by the cells. The presence of the intracellular calcium chelator BAPTA protected the cells from H2O2-induced DNA damage in a dose-dependent manner. Only at the higher rate of H2O2-generation considerable DNA damage was observed in the presence of BAPTA. These results suggest that H2O2, at low concentrations induces DNA damage through intracellular Ca2+ -mediated processes, which lead to DNA strand breaks possibly by endonuclease activation.
Περιγραφή
Λέξεις-κλειδιά
Antioxidants/*pharmacology, Ascorbic Acid/pharmacology, Calcium/*blood, Catalase/pharmacology, Cells, Cultured, Cycloheximide/pharmacology, Cytochalasin B/pharmacology, DNA/drug effects, *DNA Damage, Dehydroascorbic Acid/pharmacology, Egtazic Acid/analogs & derivatives/pharmacology, Electrophoresis, Glucose Oxidase/*metabolism, Humans, Hydrogen Peroxide/*pharmacology, Kinetics, Lymphocytes/drug effects/*physiology, Oxidative Stress, Vitamin E/pharmacology
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/10218643
http://ac.els-cdn.com/S0891584998002494/1-s2.0-S0891584998002494-main.pdf?_tid=c1af29f23e884486e5425c317a7da8fe&acdnat=1337848943_7de919b7451365da7e744d6597722b3a
http://ac.els-cdn.com/S0891584998002494/1-s2.0-S0891584998002494-main.pdf?_tid=c1af29f23e884486e5425c317a7da8fe&acdnat=1337848943_7de919b7451365da7e744d6597722b3a
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής