Proteinase and lipase production by Pseudomonas fluorescens. Proteolysis and lipolysis in thermized ewe's milk
Φόρτωση...
Ημερομηνία
Συγγραφείς
Matselis, E.
Roussis, I. G.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Food Control
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The effect of culture temperature and agitation and of iron and ascorbic acid addition on growth and extracellular proteinase and lipase production by a Pseudomonas fluorescens strain were studied by culturing in skim milk. Ps. fluorescens MRI produced significant amounts of lipase and proteinase during idiophase when cultures reached 10(7)-10(8) and 10(8)-10(9) cfu/ml, respectively. This strain produced similar maximal amounts of lipase at 5 or 25 degrees C while lower amounts of proteinase were produced at 5 degrees C. Culture agitation increased the ability of strain used to produce lipase and proteinase. Iron addition did not affect growth rate of Ps. fluorescens MR1. It did not affect its ability to produce proteinase, while increased its ability to produce lipase. Ascorbic acid addition limited the culture population of Ps. fluorescens MRI. Lipase activities were similar in the presence or absence of ascorbic acid, while proteinase production was low. Proteolysis and lipolysis in thermized ewe's milk during storage at 7 or 2 degrees C were also examined. Lipolysis in heat treated (63 degrees C x 60 s) ewe's milks was lower (P <0.05) than in unheated milks after 4 or 6 days of incubation at 7 or 2 degrees C Proteolysis in heated milks was also statistically lower (P < 0.05) after 6 days incubation at 7 degrees C, but not after 4-6 days incubation at 2 degrees C. The application of the above treatment before the cold storage of milk (3-4 degrees C, 72 h) followed by pasteurization (72 degrees C x 15 s) reduced lipolysis (P < 0.05) in milks incubated at 7 or 2 degrees C for 6 days. (C) 1998 Elsevier Science Ltd. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
pseudomonas fluorescens, lipase, proteinase, ewe milk, thermization, extracellular enzyme-production, psychrotrophic bacteria, raw-milk, growth, temperature, strains, iron, fat
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000076219300001
http://ac.els-cdn.com/S0956713598000103/1-s2.0-S0956713598000103-main.pdf?_tid=47a5aeb745003e2a1b800b7689574bdc&acdnat=1333038167_3c5c5dc9a78a2390158648963223c1c6
http://ac.els-cdn.com/S0956713598000103/1-s2.0-S0956713598000103-main.pdf?_tid=47a5aeb745003e2a1b800b7689574bdc&acdnat=1333038167_3c5c5dc9a78a2390158648963223c1c6
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας