CD5-positive and CD5-negative human B cells converge to an indistinguishable population on signalling through B-cell receptors and CD40

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dc.contributor.authorGagro, A.en
dc.contributor.authorMcCloskey, N.en
dc.contributor.authorChalla, A.en
dc.contributor.authorHolder, M.en
dc.contributor.authorGrafton, G.en
dc.contributor.authorPound, J. D.en
dc.contributor.authorGordon, J.en
dc.date.accessioned2015-11-24T19:31:25Z
dc.date.available2015-11-24T19:31:25Z
dc.identifier.issn0019-2805-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23261
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectAnimalsen
dc.subjectAntigens, CD40/*immunologyen
dc.subjectAntigens, CD5/*analysisen
dc.subjectB-Lymphocyte Subsets/*immunologyen
dc.subjectCell Culture Techniquesen
dc.subjectCell Cycle/immunologyen
dc.subjectCell Division/immunologyen
dc.subjectDNA/biosynthesisen
dc.subjectFetal Blood/immunologyen
dc.subjectHumansen
dc.subjectImmunophenotypingen
dc.subjectInfant, Newbornen
dc.subjectLymphocyte Activation/immunologyen
dc.subjectMiceen
dc.subjectPalatine Tonsil/immunologyen
dc.subjectReceptors, Antigen, B-Cell/*immunologyen
dc.subjectSignal Transduction/*immunologyen
dc.titleCD5-positive and CD5-negative human B cells converge to an indistinguishable population on signalling through B-cell receptors and CD40en
heal.abstractWhether CD5 on B cells marks a subset functionally distinct from the conventional CD5 negative (CD5neg) adult population or is more an indicator of activation, remains contentious. Here we have investigated whether CD5 positive (CD5pos) and CD5neg B cells can be distinguished in terms of their response to surrogate signals aimed to model, in vitro, T-cell dependent (TD) and T-independent (TI) encounters with antigen in vivo: the predominantly CD5pos B-cell population found in cord blood, CD5 B cells positively selected from tonsils and their CD5neg counterparts, were compared. Neonatal B cells displayed a near-identical phenotype to that of adult CD5pos B cells, being characterized by uniform immunoglobulin M (IgM), immunoglobulin D (IgD), CD23 and CD44 coexpression. When cultured with anti-IgM maintained at high density on CD32-tranfected mouse L cells to model TI responses or on CD40 ligand (CD40L)-bearing L cells (with or without captured anti-IgM) to model TD encounters, DNA synthesis was stimulated to a similar extent in all three populations. Focusing on CD5 and CD23, we found that - although the signals delivered promoted distinct profiles of expression - under each condition of activation, the phenotypes that emerged for adult CD5pos and CD5neg B cells were remarkably similar. Neonatal B cells displayed a greater diminution in CD5 expression than adult CD5pos B cells following CD40 signals but otherwise the two populations again behaved similarly. The inclusion of interleukin-4 (IL-4) to cultures where cells were costimulated via surface (s)IgM and CD40 resulted in a complete loss of CD5 expression and a corresponding hyperexpression of CD23, irrespective of the population studied. The near-identical response of CD5pos and CD5neg B cells to surrogate TD or TI signals in vitro and their convergence to indistinguishable phenotypes is wholly supportive of CD5 being a fluctuating marker of activation rather than it delineating functionally distinct subsets.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11012773-
heal.journalNameImmunologyen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2000-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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