Adenovirus-mediated expression of antisense MMP-9 in glioma cells inhibits tumor growth and invasion

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dc.contributor.authorLakka, S. S.en
dc.contributor.authorRajan, M.en
dc.contributor.authorGondi, C.en
dc.contributor.authorYanamandra, N.en
dc.contributor.authorChandrasekar, N.en
dc.contributor.authorJasti, S. L.en
dc.contributor.authorAdachi, Y.en
dc.contributor.authorSiddique, K.en
dc.contributor.authorGujrati, M.en
dc.contributor.authorOlivero, W.en
dc.contributor.authorDinh, D. H.en
dc.contributor.authorKouraklis, G.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorRao, J. S.en
dc.date.accessioned2015-11-24T19:37:37Z
dc.date.available2015-11-24T19:37:37Z
dc.identifier.issn0950-9232-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/24056
dc.rightsDefault Licence-
dc.subjectAdenoviridae/*geneticsen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectBrain Neoplasms/enzymology/*genetics/pathologyen
dc.subjectCell Division/*geneticsen
dc.subjectDNA Primersen
dc.subjectGlioma/enzymology/*genetics/pathologyen
dc.subjectMatrix Metalloproteinase 9/*geneticsen
dc.subjectMiceen
dc.subjectNeoplasm Invasiveness/*geneticsen
dc.subjectRatsen
dc.subjectTumor Cells, Cultureden
dc.titleAdenovirus-mediated expression of antisense MMP-9 in glioma cells inhibits tumor growth and invasionen
heal.abstractMatrix metalloproteinase 9 (MMP-9) is known to play a major role in cell migration and invasion in both physiological and pathological processes. Our previous work has shown that increased MMP-9 levels are associated with human glioma tumor progression. In this study, we evaluated the ability of an adenovirus containing a 528 bp cDNA sequence in antisense orientation to the 5' end of the human MMP-9 gene (Ad-MMP-9AS) to inhibit the invasiveness and migratory capacity of the human glioblastoma cell line SBN19 in in vitro and in vivo models. Infection of glioma cells with Ad-MMP-9AS reduced MMP-9 enzyme activity by approximately 90% compared with mock- or Ad-CMV-infected cells. Migration and invasion of glioblastoma cells infected with Ad-MMP-9AS were significantly inhibited relative to Ad-CMV-infected controls in spheroid and Matrigel assays. Intracranial injections of SNB19 cells infected with Ad-MMP-9AS did not produce tumors in nude mice. However, injecting the Ad-MMP-9AS construct into subcutaneous U87MG tumors in nude mice caused regression of tumor growth. These results support the theory that adenoviral-mediated delivery of the MMP-9 gene in the antisense orientation has therapeutic potential for treating gliomas.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1038/sj.onc.1205894-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/12439751-
heal.identifier.secondaryhttp://www.nature.com/onc/journal/v21/n52/pdf/1205894a.pdf-
heal.journalNameOncogeneen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2002-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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