Isolation of microbial pathogens of subclinical mastitis from raw sheep's milk of Epirus (Greece) and their role in its hygiene
Φόρτωση...
Ημερομηνία
Συγγραφείς
Fotou, K.
Tzora, A.
Voidarou, C.
Alexopoulos, A.
Plessas, S.
Avgeris, I.
Bezirtzoglou, E.
Akrida-Demertzi, K.
Demertzis, P. G.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier Masson
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Anaerobe
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The natural raw milk microflora is a factor that expresses its sensorial characteristics. The microbial charge into the mammary gland of healthy animal is low and the application of right and healthy conditions during milking and cheese making procedure, prevents from contaminating as well as maintains the natural microflora in order to lend the particular characteristics of milk. The purpose of the present project was the study of the Total Viable Count (T.V.C.) and the count of total psychrotropic bacteria of raw sheep milk from Boutsiko and Karamaniko breeds, collected from healthy animals, as well as the isolation, identification and enumeration of pathogenic bacteria related with the hygiene and the quality of raw sheep milk (with a particular interest in bacteria that may cause human infection). During the experiment we examined two hundred forty (240) samples of raw sheep milk. In these samples a) Staphylococcus aureus, Salmonella sp., Escherichia coli, Clostridium perfringens (vegetative cells and spores) and Bacillus sp. were isolated and identified b) the Total Viable Count and the total number of psychrotropic bacteria were also specified. The sampling, the preparation of samples and decimal dilutions were based on international methods. The Total viable count was determined using the standard methods of the American Public Health Association, 2002. The total number of psychrotropic bacteria was determined using APHA 1976, 1978 rules. The identification of the bacteria was carried out according to the Bergey's manual. Microscopic examination of Gram stained cells, catalase, oxidase and biochemical tests were performed when necessary to further identify. From the 240 milk samples tested, only 5% were E. coli positive, with mean counts ranged from 2 x 103 to 2.4 x 10(4) cfu/ml. S. aureus was isolated from 24% of the samples and the mean count per ml was ranged from <10 to 3.4 x 10(2). Meanwhile, Bacillus spp. was also detected in 29% samples. Vegetative forms and spores of C. perfringens were detected in 13% and 63% of the samples respectively. However, microbiological analyses revealed the presence of a small number of selected pathogens in milk samples such as Salmonella, which was only detected in 5% of the samples. Listeria sp., Pseudomonas sp. and Vibrio cholerae were never found. From the experimental results, the Total Viable Count from raw sheep milk samples, fulfils the microbiological criteria of EU Legislation in a percentage of approximately 97%. (C) 2011 Elsevier Ltd. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
raw sheep milk, staphylococcus aureus, salmonella sp, clostridium perfringens, total viable count, total number of psychrotropic bacteria, dairy-products, staphylococcus-aureus, microbiological quality, enterotoxin-a, cheese, microorganisms, psychrotrophs, outbreak, goats, area
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000299982800016
http://ac.els-cdn.com/S1075996411000837/1-s2.0-S1075996411000837-main.pdf?_tid=3aff8ef6-37c9-11e3-a90e-00000aab0f6b&acdnat=1382082497_493e8602d0a842d6517d3d51235b12e0
http://ac.els-cdn.com/S1075996411000837/1-s2.0-S1075996411000837-main.pdf?_tid=3aff8ef6-37c9-11e3-a90e-00000aab0f6b&acdnat=1382082497_493e8602d0a842d6517d3d51235b12e0
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας