Implication of lipoprotein associated phospholipase A(2) activity in oxLDL uptake by macrophages
Φόρτωση...
Ημερομηνία
Συγγραφείς
Markakis, K. P.
Koropouli, M. K.
Grammenou-Savvoglou, S.
van Winden, E. C.
Dimitriou, A. A.
Demopoulos, C. A.
Tselepis, A. D.
Kotsifaki, E. E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Lipid Research
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Recognition and uptake of oxidized LDL (oxLDL) by scavenger receptors of macrophages and foam cell formation are mediated by the oxidatively modified apolipoprotein B (ApoB) and lipid moiety of oxLDL. A great amount of oxidized phosphatidylcholine (oxPC) of oxLDL is hydrolyzed at the sn-2 position by lipoprotein associated phospholipase A(2) (Lp-PLA(2)) to lysophosphatidylcholine and small oxidation products. This study examines the involvement of Lp-PLA(2) in the uptake of oxLDL by mouse peritoneal macrophages. LDL with intact Lp-PLA(2) activity [LDL (+)] and LDL with completely inhibited Lp-PLA(2) activity [LDL (-)] were subjected to oxidation with 5 mu M CuSO(4) for 6 h [moderately oxLDL (MoxLDL)], or 24 h [heavily oxLDL (HoxLDL)] and peritoneal macrophages were incubated with these preparations. The uptake of MoxLDL(-) was about 30% increased compared with that of MoxLDL(+), and HoxLDL(-) uptake was about 20% increased compared with that of HoxLDL(+). Inhibition of Lp-PLA(2) activity had no effect on the uptake of ApoB-liposomes conjugates with ApoB isolated from MoxLDL(-), MoxLDL(+), HoxLDL(-), and HoxLDL(+). Liposomes prepared from the lipid extract of MoxLDL(-), MoxLDL(+), HoxLDL(-), and HoxLDL(+) exhibited a similar pattern to that observed in the uptake of the corresponding intact lipoproteins.jlr This study suggests that the progressive inactivation of Lp-PLA(2) during LDL oxidation leads to an increased uptake of oxLDL by macrophages, which could be primarily attributed to the increased uptake of the oxidized phospholipids enriched lipid moiety of oxLDL.-Markakis, K. P., M. K. Koropouli, S. Grammenou-Savvoglou, E. C. van Winden, A. A. Dimitriou, C. A. Demopoulos, A. D. Tselepis, and E. E. Kotsifaki. Implication of lipoprotein associated phospholipase A(2) activity in oxLDL uptake by macrophages. J. Lipid Res. 2010. 51: 2191-2201.
Περιγραφή
Λέξεις-κλειδιά
oxidized 1-palmitoyl-2-arachidonoyl-phosphatidylcholine, lysophosphatidylcholine, moderately oxidized ldl, heavily oxidized ldl, low-density-lipoprotein, activating-factor-acetylhydrolase, scavenger receptor cd36, foam cell-formation, oxidized phospholipids, apolipoprotein-b, human-plasma, atherosclerotic lesions, independent predictor, protein moieties
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000279896800015
http://www.jlr.org/content/51/8/2191.full.pdf
http://www.jlr.org/content/51/8/2191.full.pdf
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας