Mild stretch activates cPLA(2) in alveolar type II epithelial cells independently through the MEK/ERK and PI3K pathways
| dc.contributor.author | Letsiou, E. | en |
| dc.contributor.author | Kitsiouli, E. | en |
| dc.contributor.author | Nakos, G. | en |
| dc.contributor.author | Lekka, M. E. | en |
| dc.date.accessioned | 2015-11-24T16:41:53Z | |
| dc.date.available | 2015-11-24T16:41:53Z | |
| dc.identifier.issn | 1388-1981 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/8484 | |
| dc.rights | Default Licence | - |
| dc.subject | mechanical stretch | en |
| dc.subject | a549 cells | en |
| dc.subject | cytosolic pla(2) | en |
| dc.subject | erk1/2 | en |
| dc.subject | pi3 kinase | en |
| dc.subject | alveolar epithelial cells | en |
| dc.subject | cytosolic phospholipase a(2) | en |
| dc.subject | protein-kinase kinase | en |
| dc.subject | induced lung injury | en |
| dc.subject | surfactant secretion | en |
| dc.subject | arachidonic-acid | en |
| dc.subject | map kinase | en |
| dc.subject | in-vitro | en |
| dc.subject | inhibitor | en |
| dc.subject | apoptosis | en |
| dc.subject | release | en |
| dc.title | Mild stretch activates cPLA(2) in alveolar type II epithelial cells independently through the MEK/ERK and PI3K pathways | en |
| heal.abstract | Alveolar epithelial type II cells (AT II) in which lung surfactant synthesis and secretion take place, are subjected to low magnitude stretch during normal breathing. The aim of the study was to explore the effect of mild stretch on phospholipase A(2) (PLA(2)) activation, an enzyme known to be involved in surfactant secretion. In A549 cells (a model of AT II cells), we showed, using a fluorometric assay, that stretch triggers an increase of total PLA(2) activity. Western blot experiments revealed that the cytosolic isoform cPLA(2) is rapidly phosphorylated under stretch, in addition to a modest increase in cPLA(2) mRNA levels. Treatment of A549 cells with selective inhibitors of the MEK/ERK pathway significantly attenuated the stretch-induced cPLA(2) phosphotylation. A strong interaction of cPLA(2) and pERK enzymes was demonstrated by immunoprecipitation. We also found that inhibition of PI3K pathway attenuated cPLA(2) activation after stretch, without affecting pERK levels. Our results suggest that low magnitude stretch can induce cPLA(2) phosphorylation through the MEK/ERK and PI3K-Akt pathways, independently. (C) 2010 Elsevier B.V. All rights reserved. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | DOI 10.1016/j.bbalip.2010.12.007 | - |
| heal.identifier.secondary | <Go to ISI>://000291192600002 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S1388198110002556/1-s2.0-S1388198110002556-main.pdf?_tid=0eb6a866b490af30cb571543ab478662&acdnat=1333033907_1199deab89a7dc93c432e4cd67896c78 | - |
| heal.journalName | Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2011 | - |
| heal.publisher | Elsevier | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
Αρχεία
Φάκελος/Πακέτο αδειών
1 - 1 of 1
Φόρτωση...
- Ονομα:
- license.txt
- Μέγεθος:
- 1.74 KB
- Μορφότυπο:
- Item-specific license agreed upon to submission
- Περιγραφή: