Ooplasmic injection of elongating spermatids for the treatment of non-obstructive azoospermia

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dc.contributor.authorSofikitis, N. V.en
dc.contributor.authorYamamoto, Y.en
dc.contributor.authorMiyagawa, I.en
dc.contributor.authorMekras, G.en
dc.contributor.authorMio, Y.en
dc.contributor.authorToda, T.en
dc.contributor.authorAntypas, S.en
dc.contributor.authorKawamura, H.en
dc.contributor.authorKanakas, N.en
dc.contributor.authorAntoniou, N.en
dc.contributor.authorLoutradis, D.en
dc.contributor.authorMantzavinos, T.en
dc.contributor.authorKalianidis, K.en
dc.contributor.authorAgapitos, E.en
dc.date.accessioned2015-11-24T19:00:53Z
dc.date.available2015-11-24T19:00:53Z
dc.identifier.issn0268-1161-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19611
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectBiopsyen
dc.subjectCell Nucleusen
dc.subjectCytoplasmen
dc.subjectEmbryo Transferen
dc.subjectFemaleen
dc.subjectFertilization in Vitro/*methodsen
dc.subjectHumansen
dc.subjectMaleen
dc.subject*Microinjectionsen
dc.subjectMicroscopy, Confocalen
dc.subjectOligospermia/*therapyen
dc.subjectOocytes/*ultrastructureen
dc.subjectPregnancyen
dc.subject*Spermatidsen
dc.subjectTestis/pathologyen
dc.titleOoplasmic injection of elongating spermatids for the treatment of non-obstructive azoospermiaen
heal.abstractWe applied the technique of ooplasmic elongating spermatid injection to the treatment of non-obstructive azoospermia. Mature oocytes were injected with elongating spermatids isolated from testicular biopsy material obtained from 13 non-obstructed azoospermic men. Seventy-three oocytes were successfully injected with elongating spermatids and were then cultured for 36 h. At 13 h post-injection 68 oocytes were found to be activated and 52 of them were fertilized. Forty-one 2- to 4-cell stage embryos developed from normally fertilized oocytes were transferred. At least two embryos were transferred to each female partner. Two pregnancies were achieved. Elongating spermatid injection may have a role in the treatment of non-obstructive azoospermia.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9572439-
heal.identifier.secondaryhttp://humrep.oxfordjournals.org/content/13/3/709.full.pdf-
heal.journalNameHum Reproden
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1998-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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