Genistein, a dietary-derived inhibitor of in vitro angiogenesis
Φόρτωση...
Ημερομηνία
Συγγραφείς
Fotsis, T.
Pepper, M.
Adlercreutz, H.
Fleischmann, G.
Hase, T.
Montesano, R.
Schweigerer, L.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Proc Natl Acad Sci U S A
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Consumption of a plant-based diet can prevent the development and progression of chronic diseases that are associated with extensive neovascularization; however, little is known about the mechanisms. To determine whether prevention might be associated with dietary-derived angiogenesis inhibitors, we have fractionated urine of healthy human subjects consuming a plant-based diet and examined the fractions for their abilities to inhibit the proliferation of vascular endothelial cells. Using gas chromatography-mass spectrometry, we showed that one of the most potent fractions contained several isoflavonoids, which we subsequently synthesized. Of all synthetic compounds, the isoflavonoid genistein was the most potent and inhibited endothelial cell proliferation and in vitro angiogenesis at concentrations giving half-maximal inhibition of 5 and 150 microM, respectively. As we have previously demonstrated, genistein concentrations in urine of subjects consuming a plant-based diet are in the micromolar range, while those of subjects consuming a traditional Western diet are lower by a factor of > 30. The high excretion of genistein in urine of vegetarians and our present results suggest that genistein may contribute to the preventive effect of a plant-based diet on chronic diseases, including solid tumors, by inhibiting neovascularization. Thus, genistein may represent a member of a new class of dietary-derived anti-angiogenic compounds.
Περιγραφή
Λέξεις-κλειδιά
Adrenal Cortex/blood supply, Animals, Capillaries, Cattle, Cell Division/drug effects, Cells, Cultured, Cerebrovascular Circulation, Chromatography, Ion Exchange, Clone Cells, *Diet, Vegetarian, Endothelium, Vascular/cytology/*drug effects, Fibroblast Growth Factor 2/pharmacology, Gas Chromatography-Mass Spectrometry, Genistein, Growth Inhibitors/pharmacology/*urine, Humans, Isoflavones/isolation & purification/*pharmacology/*urine, Molecular Structure, *Neovascularization, Pathologic, Soybeans
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/7681986
http://www.pnas.org/content/90/7/2690.full.pdf
http://www.pnas.org/content/90/7/2690.full.pdf
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής