Hydrolysis by phospholipase D of phospholipids in solution state or adsorbed on a silica matrix
Φόρτωση...
Ημερομηνία
Συγγραφείς
Pantazi, D.
Drougas, E.
Loppinet, B.
Tellis, C.
Kosmas, A. M.
Lekka, M. E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Chemistry and Physics of Lipids
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
P hospholipases D (PLD) catalyse hydrolysis and transphosphatidylation reactions in phospholipids. In the present study, the hydrolytic activity for cabbage PLD was investigated with five different substrates (dipalmitoylphosphatidylethanolamine (DPPE), dipalmitoylphosphaticlylcholine (DPPC), didecanoylphosphatidylcholine (DDPC), 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine and lyso-phosphaticlylcholine (lyso-PC)) in solution or adsorbed on a silica matrix. In the specific buffer solutions, where the substrates were proved to form large multilamellar polydisperse aggregates, PLD showed preference for DPPC > DPPE > DDPC > alkyl-2-acetyl-sn-glycero-3-phosphocholine > lyso-PC. When the substrates were adsorbed on the silica matrix, PLD hydrolysed 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine and lyso-PC, DDPC, but not DPPC or DPPE. Theoretical studies of the simplest possible adducts between the phospholipids and the silica matrix were performed. Examination of local geometries of DPPC showed a significant blocking of the P-O-X bond-prone to hydrolysis, which could possibly block the access of PLD. Immobilization of phospholipids could be applied for improving the yield of reactions catalysed by PLD as well as for performing a targeted production of short-chain length phosphatidic acid analogs. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
phospholipase d, phospholipids, phosphatidic acid, phosphatidylcholine, silica matrix, structure calculations, multiple forms, cabbage, transphosphatidylation, phosphatidylcholine, chromatography, purification, substrate, density, model
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000234822000003
http://ac.els-cdn.com/S0009308405001453/1-s2.0-S0009308405001453-main.pdf?_tid=e763dc1bff9c223e0802a5613d8c6121&acdnat=1333034010_eb9c17a51dc58295ad79203bea3f01af
http://ac.els-cdn.com/S0009308405001453/1-s2.0-S0009308405001453-main.pdf?_tid=e763dc1bff9c223e0802a5613d8c6121&acdnat=1333034010_eb9c17a51dc58295ad79203bea3f01af
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας