A peptide carrier with a built-in vaccine adjuvant: Construction of immunogenic conjugates
| dc.contributor.author | Krikorian, D. | en |
| dc.contributor.author | Panou-Pomonis, E. | en |
| dc.contributor.author | Voitharou, C. | en |
| dc.contributor.author | Sakarellos, C. | en |
| dc.contributor.author | Sakarellos-Daitsiotis, M. | en |
| dc.date.accessioned | 2015-11-24T16:58:06Z | |
| dc.date.available | 2015-11-24T16:58:06Z | |
| dc.identifier.issn | 1043-1802 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10656 | |
| dc.rights | Default Licence | - |
| dc.subject | sequential oligopeptide carriers | en |
| dc.subject | t-cell epitopes | en |
| dc.subject | circular-dichroism | en |
| dc.subject | antigenic peptides | en |
| dc.subject | immune-responses | en |
| dc.subject | b-cell | en |
| dc.subject | autoantibodies | en |
| dc.subject | determinants | en |
| dc.subject | proteins | en |
| dc.subject | diversification | en |
| dc.title | A peptide carrier with a built-in vaccine adjuvant: Construction of immunogenic conjugates | en |
| heal.abstract | A multifunctional carrier combining B/T cell epitopes (i), a built-in vaccine adjuvant (ii), and a universal T cell epitope (iii) for the construction of potent and specific immunogenic conjugates is presented. The IL-1 beta(163-171) fragment known to reproduce the immunostimulatory and adjuvant effects of the whole IL-1 beta without possessing any of the pro-inflammatory properties of IL-1 beta was covalently anchored to the N-terminus of the Sequential Oligopeptide Carrier, SOCn, formed by the repeating tripeptide unit Lys-Aib-Gly. A promiscuous T cell epitope derived from the tetanus toxin, TT(593599), was also positioned in the carboxy terminus of SOCn, as a universal immunogen to provide broad immunogenicity. Selected B/T cell epitopes from the Sm and La/SSB autoantigens, against which is directed the humoral autoimmunity in patients with systemic lupus erythematosus and Sjogren's Syndrome, respectively, were coupled to the Lys-(NH2)-H-epsilon groups of the carrier, and the formulated constructs were administered in animals following the conventional immunization protocol of complete/incomplete Freund's adjuvant. The induced immune responses were compared with that produced when the Sm- and La/SSB-reconstituted immunogenic conjugates were injected alone. High titers of specific antibodies recognizing the priming construct, as well as the cognate autoantigen, were obtained when administered alone without the assistance of Freund's adjuvant. It is concluded that our approach provides the conceptual and experimental framework for the development of multifunctional immunogenic conjugates eliciting enhanced, specific, and prolonged humoral response for usage as human vaccine candidates. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | Doi 10.1021/Bc049703m | - |
| heal.identifier.secondary | <Go to ISI>://000230712900009 | - |
| heal.identifier.secondary | http://pubs.acs.org/doi/abs/10.1021/bc049703m | - |
| heal.journalName | Bioconjugate Chemistry | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2005 | - |
| heal.publisher | American Chemical Society | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
Αρχεία
Φάκελος/Πακέτο αδειών
1 - 1 of 1
Φόρτωση...
- Ονομα:
- license.txt
- Μέγεθος:
- 1.74 KB
- Μορφότυπο:
- Item-specific license agreed upon to submission
- Περιγραφή: