Protection against nuclear DNA damage offered by flavonoids in cells exposed to hydrogen peroxide: The role of iron chelation
Φόρτωση...
Ημερομηνία
Συγγραφείς
Melidou, M.
Riganakos, K.
Galaris, D.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Free Radical Biology and Medicine
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The ability of a number of flavonoids belonging to the flavone, flavonol, flavanone, and flavan-3-ol subclasses to protect cellular DNA from H2O2-induced single-strand breaks and the underlying molecular mechanisms were investigated in this work. Formation of single-strand breaks on nuclear DNA, after exposure of Jurkat cells to continuously generated H2O2 in the presence or absence of the flavonoid compounds, was evaluated by the comet assay (single-cell gel electrophoresis). The results indicate the following structural requirements of flavonoids for effective DNA protection: (a) the ortho-dihydroxy structure in either ring A or ring B, (b) the hydroxyl moiety on position 3 in combination with the oxo group at position 4, and (c) the presence of a C-2, C-3 double bond in ring C. In contrast to free flavonoids, the ability of complexes of [Fe2+]/[flavonoid] to protect nuclear DNA was decreased as the ratio increased, and the complex was completely inactive when the ratio reached a certain value. Moreover, it was observed that several of the flavonoids tested were able to remove iron from calcein loaded into cells and that this property was in excellent correlation with their ability to protect DNA (Speartnan's correlation coefficient, p = 0.9, p = 0.005). The antioxidant (electron donating) capacities of the same flavonoids were also evaluated by a conventional method, but no relation with their DNA-protective ability could be established even when their membrane-penetrating abilities were taken into account (p = 0.64). In conclusion, the results presented in this work strongly support the notion that intracellular binding of iron is responsible for the protection offered by flavonoids against H2O2-induced DNA damage. (c) 2005 Elsevier Inc. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
calcein, comet assay, DNA damage, flavonoids, hydrogen peroxide, iron chelation, free radicals, antioxidant activity relationships, oxidative damage, mammalian-cells, in-vitro, prevention, quercetin, apoptosis, proliferation, angiogenesis, fibroblasts
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000233847700006
http://ac.els-cdn.com/S0891584905004405/1-s2.0-S0891584905004405-main.pdf?_tid=3ccb7660a058831261004887fa468264&acdnat=1333037644_3a933744e44191723dad4d58b93f71a9
http://ac.els-cdn.com/S0891584905004405/1-s2.0-S0891584905004405-main.pdf?_tid=3ccb7660a058831261004887fa468264&acdnat=1333037644_3a933744e44191723dad4d58b93f71a9
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας