Effects of 3-methylcholanthrene and aspirin co-administration on ALDH3A1 in HepG2 cells
| dc.contributor.author | Sotiropoulou, M. | en |
| dc.contributor.author | Pappas, P. | en |
| dc.contributor.author | Marselos, M. | en |
| dc.date.accessioned | 2015-11-24T19:21:56Z | |
| dc.date.available | 2015-11-24T19:21:56Z | |
| dc.identifier.issn | 0009-2797 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/22132 | |
| dc.rights | Default Licence | - |
| dc.subject | Aldehyde Dehydrogenase/*metabolism | en |
| dc.subject | Aspirin/*administration & dosage | en |
| dc.subject | Carcinoma, Hepatocellular/*drug therapy/*enzymology/pathology | en |
| dc.subject | Cell Survival/drug effects | en |
| dc.subject | Drug Interactions | en |
| dc.subject | Glutathione Transferase/metabolism | en |
| dc.subject | Humans | en |
| dc.subject | Liver Neoplasms/*drug therapy/*enzymology/pathology | en |
| dc.subject | Methylcholanthrene/*administration & dosage | en |
| dc.subject | Tumor Cells, Cultured | en |
| dc.title | Effects of 3-methylcholanthrene and aspirin co-administration on ALDH3A1 in HepG2 cells | en |
| heal.abstract | The effects of two different protocols of 3-methylcholanthrene (3MC) and aspirin co-administration were studied in a well-established human hepatoma cell line (HepG2). During this work, we have performed toxicity tests for cell viability/cell proliferation as well as studies on the expression of ALDH3A1 after exposure of HepG2 cells to 3MC or/and aspirin. For the evaluation of toxic concentrations of 3MC and aspirin, the WST-1 test was used. WST-1 is a reliable cytotoxicity test which is based on the cleavage of the tetrazolium salt WST-1 to formazan by mitochondrial enzymes of living cells. A broad range of drug concentrations for either 3MC (0.25-50.0 microM) or aspirin (0.05-10.0 mM) were used for cell exposure, in several periods of time. The expression of ALDH3A1 in HepG2 cells showed typical time- and dose-response curves of induction after application of 3MC (1-5 days, 1.5-5.0 microM, respectively). When cells were firstly exposed to 3MC (2.5 and 5.0 microM) and then to aspirin (0.25 mM), the induced ALDH3A1 activity was further enhanced in a statistically significant way (P<0.05). On the contrary, when aspirin application was preceded 3MC exposuring a statistically significant decrease in ALDH3A1 inducibility was observed, as compared with the application of 3MC alone. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/11306048 | - |
| heal.journalName | Chem Biol Interact | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2001 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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