DNA protecting and genotoxic effects of olive oil related components in cells exposed to hydrogen peroxide

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dc.contributor.authorNousis, L.en
dc.contributor.authorDoulias, P. T.en
dc.contributor.authorAligiannis, N.en
dc.contributor.authorBazios, D.en
dc.contributor.authorAgalias, A.en
dc.contributor.authorGalaris, D.en
dc.contributor.authorMitakou, S.en
dc.date.accessioned2015-11-24T19:23:59Z
dc.date.available2015-11-24T19:23:59Z
dc.identifier.issn1071-5762-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22398
dc.rightsDefault Licence-
dc.subjectCaffeic Acids/isolation & purification/pharmacologyen
dc.subjectChromatography, High Pressure Liquiden
dc.subjectComet Assayen
dc.subjectDNA/*drug effects/metabolismen
dc.subject*DNA Damageen
dc.subjectHumansen
dc.subjectHydrogen Peroxide/*toxicityen
dc.subjectJurkat Cells/drug effectsen
dc.subjectNuclear Magnetic Resonance, Biomolecularen
dc.subjectOlea/chemistryen
dc.subjectPhenylethyl Alcohol/analogs & derivatives/isolation & purification/pharmacologyen
dc.subjectPlant Extracts/chemistry/pharmacologyen
dc.subjectPlant Oils/*chemistry/*pharmacologyen
dc.subjectPyrans/isolation & purification/pharmacologyen
dc.titleDNA protecting and genotoxic effects of olive oil related components in cells exposed to hydrogen peroxideen
heal.abstractIn search for compounds, able to protect nuclear DNA in cells exposed to oxidative stress, extracts from olive leaves, olive fruits, olive oil and olive mill waste water were tested by using the "single cell gel electrophoresis" methodology (comet assay). Jurkat cells in culture were exposed to continuously generated hydrogen peroxide (11.8+/-1.5 microM per min) by direct addition into the growth medium of the appropriate amount of the enzyme "glucose oxidase" in the presence or absence of the tested total extracts. The protective effects of the tested extracts or isolated compounds were evaluated from their ability to decrease hydrogen peroxide-induced formation of single strand breaks in the nuclear DNA, while the toxic effects were estimated from the increase of DNA damage when the extracts or isolated compounds were incubated directly with the cells. Significant protection was observed in extracts from olive oil and olive mill waste water. However, above a concentration of 100 microg/ml olive oil extracts exerted DNA damaging effects by themselves in the absence of any H2O2. Extracts from olive leaves and olive fruits although protective, were also able to induce DNA damage by themselves. Main compounds isolated from the above described total extracts, like oleuropein glucoside, tyrosol, hydroxytyrosol and caffeic acid, were tested in the same experimental system and found to exert cytotoxic (oleuropein glucoside), no effect (tyrosol) or protective effects (hydroxytyrosol and caffeic acid). In conclusion, cytoprotective as well as cytotoxic compounds with potential pharmaceutical properties were detected in extracts from olive oil related sources by using the comet assay methodology.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1080/10715760500045806-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/16036359-
heal.identifier.secondaryhttp://informahealthcare.com/doi/pdfplus/10.1080/10715760500045806-
heal.journalNameFree Radic Resen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2005-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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