Effects of primary testicular damage on sperm DNA oxidative status and embryonic and foetal development
Φόρτωση...
Ημερομηνία
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Andrologia
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
We evaluated the development of embryos generated from the fertilisation of oocytes with spermatozoa isolated from animals with primary testicular damage (PTD). Embryos derived in vivo or in vitro from oocytes fertilised with spermatozoa produced by PTD rats that had undergone surgical treatment for the PTD (group A1), or PTD rats (group A2), or control rats (group B) were cultured and transferred to recipients. At the end of the experimental period, the fertilisation potential of each rat was assessed in vitro (IVF trials). Sperm 8-oxodG/dG ratio (a marker of DNA oxidative status) was significantly larger in group A2 than in groups A1 and B. Blastocysts of the group A2 transferred to recipients demonstrated a significantly larger loss before implantation than transferred blastocysts of groups A1 or B. In addition, the proportion of implanted blastocysts that could not complete the intrauterine development was significantly larger in group A2 than in groups A1 and B. This study reveals a post-fertilisation detrimental effect in animals with PTD on the capacity of oocytes (fertilised either in vitro or in vivo) to develop in vitro and implant after transferring them to recipients probably attributable to sperm DNA oxidative damage.
Περιγραφή
Λέξεις-κλειδιά
Animals, Body Temperature, *DNA Damage, Deoxyguanosine/analogs & derivatives/analysis, *Embryonic Development, Epididymis/anatomy & histology, Fertilization in Vitro, *Fetal Development, Male, Organ Size, Rats, Seminal Vesicles/anatomy & histology, Sperm Count, Sperm Motility, Spermatozoa/chemistry/*pathology, Testis/anatomy & histology/*blood supply/*injuries/surgery, Testosterone/blood, Veins
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/19737276
http://onlinelibrary.wiley.com/store/10.1111/j.1439-0272.2009.00929.x/asset/j.1439-0272.2009.00929.x.pdf?v=1&t=h0p8a4uc&s=316ff8130d1b9ef19297d740c074c35049bc9456
http://onlinelibrary.wiley.com/store/10.1111/j.1439-0272.2009.00929.x/asset/j.1439-0272.2009.00929.x.pdf?v=1&t=h0p8a4uc&s=316ff8130d1b9ef19297d740c074c35049bc9456
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής