Analysis of motile oligodendrocyte precursor cells in vitro and in brain slices
Φόρτωση...
Ημερομηνία
Συγγραφείς
Schmidt, C.
Ohlemeyer, C.
Labrakakis, C.
Walter, T.
Kettenmann, H.
Schnitzer, J.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Glia
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Oligodendrocyte precursor cells are purported to migrate over long distances into the various brain regions where they differentiate into oligodendrocytes and fulfill their appropriate tasks, i.e., myelination of axons. Here we characterize motile oligodendrocyte precursor cells in detail. Video-time lapse analysis was performed on isolated precursor cells in single cell cultures, in co-culture with cerebellar microexplants, and in living brain slices. Motility analysis of individual cells was combined with electrophysiological, immunological, and morphological characterizations. Translocation of the cell bodies was not continuous but occurred in waves. All motile cells exhibited a simple morphology and most, but not all, of them expressed the A2B5 epitope in vitro. Patch clamp analysis of the motile cells confirmed that they belong to the O-2A lineage. The percentage of motile cells, as well as their velocities, were enhanced on substrate-coated laminin in comparison to poly-L-lysine. Motility was not influenced by the presence of cerebellar microexplants. O-2A progenitor cells did not migrate strictly along neurite fascicles which were projected from the microexplants. Glial progenitor cells in situ also did not strictly migrate along the main direction of the axonal fibers of the corpus callosum but rather traversed the fibers with an overall direction toward the cortex. After Lucifer Yellow filling of the motile progenitor cells in situ, we could demonstrate that they were dye-coupled to yet unidentified cells of the corpus callosum.
Περιγραφή
Λέξεις-κλειδιά
Animals, Animals, Newborn, Brain/*cytology, Cell Movement/*physiology, Cells, Cultured, Cerebellum/cytology, Electrophysiology, Immunohistochemistry, Laminin/pharmacology, Membrane Potentials, Oligodendroglia/*physiology, Patch-Clamp Techniques, Rats, R
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/9262233
http://onlinelibrary.wiley.com/store/10.1002/(SICI)1098-1136(199708)20:4<284::AID-GLIA2>3.0.CO;2-6/asset/2_ftp.pdf?v=1&t=hh1pvw2j&s=bf0629fde1a08611b8cdc88a24f711568128b287
http://onlinelibrary.wiley.com/store/10.1002/(SICI)1098-1136(199708)20:4<284::AID-GLIA2>3.0.CO;2-6/asset/2_ftp.pdf?v=1&t=hh1pvw2j&s=bf0629fde1a08611b8cdc88a24f711568128b287
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών