Determination of plasma, urine, and bovine serum albumin low-molecular-weight carbonyl levels by capillary gas chromatography with electron-capture and mass-selective detection

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dc.contributor.authorKonidari, C. N.en
dc.contributor.authorGiannopoulos, T. S.en
dc.contributor.authorNanos, C. G.en
dc.contributor.authorStalikas, C. D.en
dc.date.accessioned2015-11-24T16:55:44Z
dc.date.available2015-11-24T16:55:44Z
dc.identifier.issn0003-2697-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/10350
dc.rightsDefault Licence-
dc.subjectlow-molecular-weight carbonyl compoundsen
dc.subjectbiological samplesen
dc.subject2,4,6-trichlorophenylhydrazineen
dc.subjectgas chromatographyen
dc.subjectlow-density lipoproteinsen
dc.subjectperformance liquid-chromatographyen
dc.subjectlipid-peroxidation productsen
dc.subjecthydroxy fatty-acidsen
dc.subjectbiological samplesen
dc.subjectfree malonaldehydeen
dc.subjectprotein oxidationen
dc.subjectlinoleic-aciden
dc.subjectmalondialdehydeen
dc.subjectspectrometryen
dc.titleDetermination of plasma, urine, and bovine serum albumin low-molecular-weight carbonyl levels by capillary gas chromatography with electron-capture and mass-selective detectionen
heal.abstractPeroxidation of lipids produces low-molecular-weight carbonyl compounds, which are reactive with biological nucleophiles. The analysis of these compounds is often difficult. A multicomponent method for the determination of 11 of them in biological samples is reported. The samples are subjected to a pretreatment-derivatization procedure followed by gas chromatographic analysis with either electron-capture detection (ECD) or mass-selective detection (MSD) in the selected-ion monitoring mode. The procedure involves derivatization of the analyte with 2,4,6-trichlorophenylhydrazine, extraction with n-hexane, and separation of the derivatization products on a nonpolar gas chromatographic column. The concentration of the derivatization reagent, pH, reaction time, temperature, and presence of extraneous ions were investigated to determine the optimal derivatization conditions. Under these conditions, the method allows for the selective detection of low-molecular-weight carbonyl compounds at femtomole levels in several biological materials such as plasma, urine, and bovine serum albumin without interferences. The limits of detection were in the ranges 0.01-0.2 muM for ECD and 0.15-1.5 muM for MSD. The mean procedural recoveries obtained during the method validation were within the range 85-95% and the intra- and interassay standard deviations do not exceed 4.6 and 6.1%, respectively. (C) 2004 Elsevier Inc. All rights reserved.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDoi 10.1016/J.Ab.2004.11.001-
heal.identifier.secondary<Go to ISI>://000227223000008-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0003269704008851/1-s2.0-S0003269704008851-main.pdf?_tid=f588ad87d28680455d2bee954f42f466&acdnat=1333040851_d403180aae8d9b34e7900f1472c8012e-
heal.journalNameAnal Biochemen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2005-
heal.publisherElsevieren
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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