The role of the chemokines MCP-1, GRO-alpha, IL-8 and their receptors in the adhesion of monocytic cells to human atherosclerotic plaques
Φόρτωση...
Ημερομηνία
Συγγραφείς
Papadopoulou, C.
Corrigall, V.
Taylor, P. R.
Poston, R. N.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Cytokine
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Monocyte adhesion to the arterial endothelium and subsequent migration into the intima are central events in the pathogenesis of atherosclerosis. Previous experimental models have shown that chemokines can enhance monocyte-endothelial adhesion by activating monocyte integrins. Our study assesses the role of chemokines IL-8, MCP-1 and GRO-alpha, together with their monocyte receptors CCR2 and CXCR2 in monocyte adhesion to human atherosclerotic plaques. In an adhesion assay, a suspension of monocytic U937 cells was incubated with human atherosclerotic artery sections and the levels of endothelial adhesion were quantified. Adhesion performed in the presence of a monoclonal antibody to a chemokine, chemokine receptor or of an isotype matched control immunoglobulin, shows that antibodies to all chemokines tested, as well as their receptors, inhibit adhesion compared to the control immunoglobulins. Immunohistochemistry demonstrated the expression of MCP-1, GRO-alpha and their receptors in the endothelial cells and intima of all atherosclerotic lesions. These results suggest that all these chemokines and their receptors can play a role in the adhesion of monocytes to human atherosclerotic plaques. Furthermore, they suggest that these chemokine interactions provide potential targets for the therapy of atherosclerosis.
Περιγραφή
Λέξεις-κλειδιά
Aged, Aged, 80 and over, Antibodies/immunology, Atherosclerosis/immunology/*metabolism/*pathology, Cell Adhesion/immunology, Cell Line, Chemokine CXCL1/immunology/metabolism, Chemokines/immunology/*metabolism, Female, Humans, Immunohistochemistry, Interleukin-8/immunology/metabolism, Male, Models, Biological, Monocytes/cytology/metabolism, Receptors, Chemokine/*metabolism
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/18579408
http://ac.els-cdn.com/S1043466608001361/1-s2.0-S1043466608001361-main.pdf?_tid=ec4104bb3fde6d95f01c80b9f97dadf9&acdnat=1332846716_025598f162d4e10da5208c55216921fb
http://ac.els-cdn.com/S1043466608001361/1-s2.0-S1043466608001361-main.pdf?_tid=ec4104bb3fde6d95f01c80b9f97dadf9&acdnat=1332846716_025598f162d4e10da5208c55216921fb
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής