The lipophilic extract of Hypericum perforatum exerts significant cytotoxic activity against T24 and NBT-II urinary bladder tumor cells
Φόρτωση...
Ημερομηνία
Συγγραφείς
Skalkos, D.
Stavropoulos, N. E.
Tsimaris, I.
Gioti, E.
Stalikas, C. D.
Nseyo, U. O.
Ioachim, E.
Agnantis, N. J.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Georg Thieme Verlag
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Planta Medica
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Hypericum perforatum L (St. John's wort) is a medicinal plant used for many pathologies, especially for the treatment of mild to moderate depression. In the present study we have investigated the cytotoxic activity of the locally collected (Epirus region) Hypericum perforatum L. against cultured T24 and NBT-II bladder cancer cell lines. The lipophilic extract of the herb, prepared using petroleum ether, induced apoptosis displaying LC50 values at concentrations as low as 4 and 5 mu g/mL. A fraction of this extract displayed 60% cell growth inhibition at a concentration of 0.95 mu g/mL. Evaluating the importance of various biologically active components of the extract, it was found that hypericins (hypericin, pseudohypericin, etc.) were identified only in the methanolic (lipophobic) extract of the herb, and not in the active lipophilic extract. In addition, hyperforin concentrations in the lipophilic extract and its most active fraction, were 0.94 mu g/mL, and 0.17 mu g/mL, respectively, while the active cytotoxic concentration of pure hyperforin appeared in the range of 1.8 mu g/mL - 5.0 mu g/mL. Therefore, pure hyperforin does not seem to contribute significantly to the cytotoxicity activity. Chlorophylls were identified in low, not significantly different, concentrations in all extracts and fractions and were not correlated to the biological activity. Owing to the combination of significant cytotoxic activity, natural abundance and low toxicity, the lipophilic extract of Hypericum perforatum holds the promise of being an interesting, new, anti proliferative agent against bladder cancer that deserves further investigation.
Περιγραφή
Λέξεις-κλειδιά
hypericum perforatum l., st. john's wort, hypericin, pseudohypericin, hyperforin, chlorophylls, bladder tumor, st-johns-wort, forced swimming test, antidepressant activity, in-vitro, cancer, hyperforin, apoptosis, constituent, flavonoids, inhibition
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000233940800008
https://www.thieme-connect.de/DOI/DOI?10.1055/s-2005-873127
https://www.thieme-connect.de/DOI/DOI?10.1055/s-2005-873127
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας