Label-free kinetic study of biomolecular interactions by white light reflectance spectroscopy
| dc.contributor.author | Zavali, M. | en |
| dc.contributor.author | Petrou, P. S. | en |
| dc.contributor.author | Kakabakos, S. E. | en |
| dc.contributor.author | Kitsara, M. | en |
| dc.contributor.author | Raptis, I. | en |
| dc.contributor.author | Beltsios, K. | en |
| dc.contributor.author | Misiakos, K. | en |
| dc.date.accessioned | 2015-11-24T17:35:18Z | |
| dc.date.available | 2015-11-24T17:35:18Z | |
| dc.identifier.issn | 1750-0443 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/14108 | |
| dc.rights | Default Licence | - |
| dc.subject | surface-plasmon resonance | en |
| dc.subject | interferometry | en |
| dc.subject | biosensor | en |
| dc.subject | adhesion | en |
| dc.subject | sensors | en |
| dc.subject | films | en |
| dc.subject | glass | en |
| dc.title | Label-free kinetic study of biomolecular interactions by white light reflectance spectroscopy | en |
| heal.abstract | Binding of biomolecules in real-time is studied by white light reflectance spectroscopy (WLRS). The experimental apparatus consists of a WLRS setup and a microfluidic channel for the supply of the biomolecules solutions placed above the sample on which the biomolecular interactions take place. Using this setup, it is possible to monitor in real-time the biomolecular film thickness changes during the binding of biomolecules on a properly treated surface. The sample is a silicon wafer with a thick thermally grown silicon dioxide (similar to 1044 nm) and a thin (similar to 35 nm) polymer layer. The proposed methodology has been applied for the monitoring of streptavidin binding to surface-immobilised biotinylated protein. The biomolecular interactions are monitored as shifts of the wavelength where constructive interference is observed. The proposed methodology provides a simple, fast, low cost approach for a label-free monitoring of biomolecular interactions. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | Doi 10.1049/Mnl:20065019 | - |
| heal.identifier.secondary | <Go to ISI>://000252698500007 | - |
| heal.journalName | Micro & Nano Letters | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2006 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Μηχανικών Επιστήμης Υλικών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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