A Zymomonas mobilis mutant with delayed growth on high glucose concentrations

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dc.contributor.authorDouka, E.en
dc.contributor.authorKoukkou, A. I.en
dc.contributor.authorVartholomatos, G.en
dc.contributor.authorFrillingos, S.en
dc.contributor.authorPapamichael, E. M.en
dc.contributor.authorDrainas, C.en
dc.date.accessioned2015-11-24T16:40:55Z
dc.date.available2015-11-24T16:40:55Z
dc.identifier.issn0021-9193-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/8333
dc.rightsDefault Licence-
dc.subjectescherichia-colien
dc.subjectpseudomonas-aeruginosaen
dc.subjectreca geneen
dc.subjecttransporten
dc.subjectexpressionen
dc.subjectethanolen
dc.subjectcloningen
dc.subjectoperonen
dc.subjectmetabolismen
dc.subjectsequenceen
dc.titleA Zymomonas mobilis mutant with delayed growth on high glucose concentrationsen
heal.abstractExponentially growing cells of Zymomonas mobilis normally exhibit a lag period of up to 3 h when transferred from 0.11 M (2%) to 0.55 M (10%) glucose liquid medium. A mutant of Z, mobilis (CU1Rif2), fortuitously isolated, showed more than a 20-h lag period when grown under the same conditions, whereas on 0.55 M glucose solid medium, it failed to grow. The growth of CU1Rif2 on elevated concentrations of other fermentable (0.55 M sucrose or fructose) or nonfermentable (0.11 M glucose plus 0.44 M maltose or xylose) sugars appeared to be normal, Surprisingly, CU1Rif2 cells grew without any delay on 0.55 M glucose on which wild-type cells had been incubated for 3 h and removed at the beginning of their exponential phase. This apparent preconditioning was not observed with medium obtained from wild-type cells grown on 0.11 M glucose and supplemented to 0.55 M after removal of the wild-type cells. Undelayed growth of CU1Rif2 on 0.55 M glucose previously conditioned by the wild type was impaired by heating or protease treatment. It is suggested that in Z, mobilis, a diffusible proteinaceous heat-labile factor, transitionally not present in 0.55 M glucose CU1Rif2 cultures, triggers growth on 0.55 M glucose. Biochemical analysis of glucose uptake and glycolytic enzymes implied that glucose assimilation was not directly involved in the phenomenon. By use of a wild-type Z. mobilis genomic library, a 4.5-kb DNA fragment which complemented in low copy number the glucose-defective phenotype as well as glucokinase and glucose uptake of CU1Rif2 was isolated. This fragment carries a gene cluster consisting of Four putative coding regions, encoding 167, 167, 145, and 220 amino acids with typical Z, mobilis codon usage, -35 and -10 promoter elements, and individual Shine-Dalgarno consensus sites. However, strong homologies were not deterred in a BLAST2 (EMBL-Heidelberg) computer search with known protein sequences.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondary<Go to ISI>://000081706100022-
heal.identifier.secondaryhttp://jb.asm.org/content/181/15/4598.full.pdf-
heal.journalNameJ Bacteriolen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate1999-
heal.publisherAmerican Society for Microbiologyen
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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