Immobilization of Glutamate Oxidase on Nonporous Glass-Beads - Automated Flow-Injection System for the Assay of Glutamic-Acid in Food Samples and Pharmaceuticals
Φόρτωση...
Ημερομηνία
Συγγραφείς
Stalikas, C. D.
Karayannis, M. I.
Tzouwarakarayanni, S. M.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Royal Society of Chemistry
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Analyst
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
An enzymic method is proposed for the determination of glutamic acid in food samples and pharmaceuticals. The method is useful in the range 0.01-0.5 mmol 1-1, with a detection limit of 0.005 mmol l-1 for an injection volume of 52 mul. L-Glutamate oxidase from Streptomyces sp. X-1 19-6 was immobilized on non-porous glass beads, and the H2O2 produced was caused to react with Trinder's reagent. Various parameters were studied for the establishment of the optimum operating conditions for an in-house flow injection manifold with a spectrophotometric detector. Many interfering species and several amino acids were tested in order to verify the specificity of the enzyme reactor. Experimental data for interfering organic and inorganic substances are reported. The system works very selectively for glutamic acid and glutamates. The method is ideally suited to the assay of glutamates in a large number of samples within a single working day, as the frequency is 40 samples h-1. The relative standard deviation is 1.8% for six assays. The immobilized reactor is stable for a long period under proper conditions of storage. The accuracy of the proposed method was tested by comparison of the results with those of the Association of Official Analytical Chemist's method and with the manufacturer's specifications for the analysed samples, where available. Good agreement was obtained. Recovery studies yielded results between 97 and 105%.
Περιγραφή
Λέξεις-κλειδιά
l-glutamic acid, glutamate oxidase, single-bead string reactor, flow injection, food and pharmaceutical samples, enzyme electrode, ascorbic-acid, interference, peroxidase, reactor
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://A1993LJ62300029
http://pubs.rsc.org/en/Content/ArticleLanding/1993/AN/an9931800723
http://pubs.rsc.org/en/Content/ArticleLanding/1993/AN/an9931800723
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας