Determination of acrylamide and methacrylamide by normal
Φόρτωση...
Ημερομηνία
Συγγραφείς
Paleologos, E. K.
Kontominas, M. G.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Chromatography A
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
A method using normal phase high performance liquid chromatography (NP-HPLC) with UV detection was developed for the analysis of acrylamide and methacrylamide. The method relies on the chromatographic separation of these analytes on a polar HPLC column designed for the separation of organic acids. Identification of acrylamide and methacrylamide is approached dually, that is directly in their protonated forms and as their hydrolysis products acrylic and methacrylic acid respectively, for confirmation. Detection and quantification is performed at 200 nm. The method is simple allowing for clear resolution of the target peaks from any interfering substances. Detection limits of 10 mu g L-1 were obtained for both analytes with the inter- and intra-day RSD for standard analysis lying below 1.0%. Use of acetonitrile in the elution solvent lowers detection limits and retention times, without impairing resolution of peaks. The method was applied for the determination of acrylamide and methacrylamide in spiked food samples without native acrylamide yielding recoveries between 95 and 103%. Finally, commercial samples of french and roasted fries, cookies, cocoa and coffee were analyzed to assess applicability of the method towards acrylamide, giving results similar with those reported in the literature. (C) 2005 Elsevier B.V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
acrylamide, methacrylamide, acrylic acid, methacrylic acid, normal phase hplc, uv detection, liquid-chromatography, mass-spectrometry, maillard reaction, foods, products, genotoxicity, carcinogen
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000229945600004
http://ac.els-cdn.com/S0021967305007958/1-s2.0-S0021967305007958-main.pdf?_tid=02d779699e0747e46c4d469839fdbfd6&acdnat=1333031370_92227a73c92084caa3770f152fa7ae8e
http://ac.els-cdn.com/S0021967305007958/1-s2.0-S0021967305007958-main.pdf?_tid=02d779699e0747e46c4d469839fdbfd6&acdnat=1333031370_92227a73c92084caa3770f152fa7ae8e
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας