Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials

Απλή σελίδα τεκμηρίου
dc.contributor.authorPavlidis, I. V.en
dc.contributor.authorVorhaben, T.en
dc.contributor.authorTsoufis, T.en
dc.contributor.authorRudolf, P.en
dc.contributor.authorBornscheuer, U. T.en
dc.contributor.authorGournis, D.en
dc.contributor.authorStamatis, H.en
dc.date.accessioned2015-11-24T17:33:13Z
dc.date.available2015-11-24T17:33:13Z
dc.identifier.issn0960-8524-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/13833
dc.rightsDefault Licence-
dc.subjectlipaseen
dc.subjectesteraseen
dc.subjectimmobilizationen
dc.subjectcarbon nanotubesen
dc.subjectgraphene oxideen
dc.subjectlipase immobilizationen
dc.subjectenzyme immobilizationen
dc.subjectsurface-chemistryen
dc.subjectnanotubesen
dc.subjectstabilityen
dc.subjectesterasesen
dc.subjectproteinsen
dc.subjectgrapheneen
dc.subjectoxideen
dc.titleDevelopment of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterialsen
heal.abstractIn this study we report the use of functionalized carbon-based nanomaterials, such as amine-functionalized graphene oxide (GO) and multi-walled carbon nanotubes (CNTs), as effective immobilization supports for various lipases and esterases of industrial interest. Structural and biochemical characterization have revealed that the curvature of the nanomaterial affect the immobilization yield, the catalytic behavior and the secondary structure of enzymes. Infrared spectroscopy study indicates that the catalytic behavior of the immobilized enzymes is correlated with their alpha-helical content. Hydrolases exhibit higher esterification activity (up to 20-fold) when immobilized on CNTs compared to GO. The covalently immobilized enzymes exhibited comparable or even higher activity compared to the physically adsorbed ones, while they presented higher operational stability. The enhanced catalytic behavior observed for most of the hydrolases covalently immobilized on amine-functionalized CNTs indicate that these functionalized nanomaterials are suitable for the development of efficient nanobiocatalytic systems. (C) 2011 Elsevier Ltd. All rights reserved.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDOI 10.1016/j.biortech.2011.11.007-
heal.identifier.secondary<Go to ISI>://000305379000027-
heal.journalNameBioresour Technolen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2012-
heal.publisherElsevieren
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Μηχανικών Επιστήμης Υλικώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

Αρχεία

Φάκελος/Πακέτο αδειών

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
license.txt
Μέγεθος:
1.74 KB
Μορφότυπο:
Item-specific license agreed upon to submission
Περιγραφή:
Κατεβάστε

Συλλογές

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)