Uncoupling protein 3 expression and intramyocellular lipid accumulation by NMR following local burn trauma

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dc.contributor.authorZhang, Q.en
dc.contributor.authorCao, H.en
dc.contributor.authorAstrakas, L. G.en
dc.contributor.authorMintzopoulos, D.en
dc.contributor.authorMindrinos, M. N.en
dc.contributor.authorSchulz, J., 3rden
dc.contributor.authorTompkins, R. G.en
dc.contributor.authorRahme, L. G.en
dc.contributor.authorTzika, A. A.en
dc.date.accessioned2015-11-24T19:00:02Z
dc.date.available2015-11-24T19:00:02Z
dc.identifier.issn1107-3756-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19465
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectBurns/*metabolism/pathologyen
dc.subjectCold Temperatureen
dc.subjectFatty Acids/analysis/metabolismen
dc.subjectHindlimb/metabolism/pathologyen
dc.subjectIon Channels/*metabolismen
dc.subject*Lipid Metabolismen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMice, Inbred Strainsen
dc.subjectMitochondrial Proteins/*metabolismen
dc.subjectMuscle, Skeletal/*chemistry/*metabolismen
dc.subject*Nuclear Magnetic Resonance, Biomolecularen
dc.subjectRNA, Messenger/metabolismen
dc.subjectTime Factorsen
dc.titleUncoupling protein 3 expression and intramyocellular lipid accumulation by NMR following local burn traumaen
heal.abstractBurn trauma is a clinical condition accompanied by muscle wasting that severely impedes rehabilitation in burn survivors. Mitochondrial uncoupling protein 3 (UCP3) is uniformly expressed in myoskeletal mitochondria and its expression has been found to increase in other clinical syndromes that, like burn trauma, are associated with muscle wasting (e.g., starvation, fasting, cancer, sepsis). The aim of this study was to explore the effects of burn trauma on UCP3 expression, intramyocellular lipids, and plasma-free fatty acids. Mice were studied at 6 h, 1 d and 3 d after nonlethal hindlimb burn trauma. Intramyocellular lipids in hindlimb skeletal muscle samples collected from burned and normal mice were measured using 1H NMR spectroscopy on a Bruker 14.1 Tesla spectrometer at 4 degrees C. UCP3 mRNA and protein levels were also measured in these samples. Plasma-free fatty acids were measured in burned and normal mice. Local burn trauma was found to result in: 1) upregulation of UCP3 mRNA and protein expression in hindlimb myoskeletal mitochondria by 6 h postburn; 2) increased intramyocellular lipids; and 3) increased plasma-free fatty acids. Our findings show that the increase in UCP3 after burn trauma may be linked to burn-induced alterations in lipid metabolism. Such a link could reveal novel insights into how processes related to energy metabolism are controlled in burn and suggest that induction of UCP3 by burn in skeletal muscle is protective by either activating cellular redox signaling and/or mitochondrial uncoupling.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/17089030-
heal.journalNameInt J Mol Meden
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2006-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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