Improved methods for automatic monitoring of contracting heart cells in culture
| dc.contributor.author | Galaris, D. | en |
| dc.contributor.author | Hoijer, B. | en |
| dc.contributor.author | Rydstrom, J. | en |
| dc.date.accessioned | 2015-11-24T19:11:24Z | |
| dc.date.available | 2015-11-24T19:11:24Z | |
| dc.identifier.issn | 0165-022X | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/20925 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Animals, Newborn | en |
| dc.subject | Autoanalysis/instrumentation/methods | en |
| dc.subject | Cells, Cultured | en |
| dc.subject | Female | en |
| dc.subject | Heart/*physiology | en |
| dc.subject | Male | en |
| dc.subject | Microbial Collagenase | en |
| dc.subject | *Myocardial Contraction | en |
| dc.subject | Rats | en |
| dc.title | Improved methods for automatic monitoring of contracting heart cells in culture | en |
| heal.abstract | The present communication describes improved methods for isolating and plating beating heart cells from neonatal rats using collagenase and collagen-coated petri dishes. The amplitude and frequency of contraction are continuously and simultaneously measured under well defined conditions and during prolonged periods of time with a highly sensitive and thermostated instrument. Additions, e.g. drugs and toxic agents, are made through an accessory pump system that involves extensive dilution of the added compound with medium; aliquots of medium can be withdrawn for estimation of metabolites. The system described is reliable and relatively inexpensive and allows a more extensive use of isolated heart cells, especially in studies of heart functions where small changes in amplitude and frequency of beating during prolonged periods of time are important. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/6252262 | - |
| heal.identifier.secondary | http://www.sciencedirect.com/science/article/pii/0165022X80900366 | - |
| heal.journalName | J Biochem Biophys Methods | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1980 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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