The effects of phosphoinositide/calcium- or cyclic AMP-mediated signal transduction pathway inhibitors on the activation of rat peritoneal macrophages by acetylated low-density lipoprotein
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Ημερομηνία
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
In Vivo
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
BACKGROUND: Macrophages that uptake modified lipoproteins are activated and may initially behave as endotoxin-stimulated macrophages. This study was undertaken in order to determine whether signal transduction pathways controlling endotoxin-mediated activation may also influence the lipoprotein-mediated activation of macrophages. MATERIALS AND METHODS: Rat peritoneal macrophages were incubated for 16 hours with acetylated low-density lipoprotein and certain agents that modify the phosphoinositide/calcium- and cyclic AMP-mediated pathways, such as 2-[4-morpholinyl]-8-phenyl-1[4H]-benzopyran-4-one (LY-294002), autocamtide 2-related inhibitory peptide (AIP), N-(2-[p-bromocinnamylamino]-ethyl)-5-isoquinolinesulfonamide hydrochloride (H-89) and actinomycin D. The production of nitric oxide and the intracellular and extracellular activities of acid phosphatase were assayed. RESULTS: Macrophages incubated with acetylated low-density lipoprotein showed an increased production of nitric oxide and intracellular acid phosphatase activity as compared to their controls. LY-294002, AIP and H-89 caused a significant decrease in nitric oxide production and intracellular acid phosphatase activity. Actinomycin D had similar effects. AIP and actinomycin also significantly increased extracellular acid phosphatase activity. CONCLUSION: The activation of peritoneal macrophages by acetylated low-density lipoprotein was similar to the activation by endotoxin, as expressed by the nitric oxide production and acid phosphatase intracellular activity; agents controlling the phosphoinositide/calcium- and cyclic AMP-mediated pathways in endotoxin-activated macrophages also influence the acetylated low-density lipoprotein-activated macrophages.
Περιγραφή
Λέξεις-κλειδιά
Acid Phosphatase/metabolism, Animals, Carrier Proteins/pharmacology, Caspase 9, Caspases/pharmacology, Cells, Cultured, Chromones/pharmacology, Cyclic AMP/metabolism, *Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors, Dactinomycin/pharmacology, Enzyme Inhibitors/*pharmacology, Female, Isoquinolines/pharmacology, Lipoproteins, LDL/*pharmacology, Macrophage Activation/*drug effects, Macrophages, Peritoneal/*drug effects/metabolism, Male, Morpholines/pharmacology, Nitric Oxide/metabolism, *Phosphatidylinositol 3-Kinases/antagonists & inhibitors, Rats, Rats, Wistar, Signal Transduction/drug effects, Specific Pathogen-Free Organisms, Sulfonamides/pharmacology
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/15523908
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής