Binding of monoclonal antibody 4B1 to homologs of the lactose permease of Escherichia coli

Απλή σελίδα τεκμηρίου
dc.contributor.authorSun, J.en
dc.contributor.authorFrillingos, S.en
dc.contributor.authorKaback, H. R.en
dc.date.accessioned2015-11-24T19:32:55Z
dc.date.available2015-11-24T19:32:55Z
dc.identifier.issn0961-8368-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23473
dc.rightsDefault Licence-
dc.subjectAmino Acid Sequenceen
dc.subjectAntibodies, Bacterialen
dc.subjectAntibodies, Monoclonalen
dc.subjectBinding Sitesen
dc.subjectBiological Transporten
dc.subjectEpitopesen
dc.subjectEscherichia coli/*enzymology/geneticsen
dc.subject*Escherichia coli Proteinsen
dc.subjectMembrane Transport Modulatorsen
dc.subjectMembrane Transport Proteins/antagonists &en
dc.subjectinhibitors/genetics/*immunology/*metabolismen
dc.subjectMembranes/metabolismen
dc.subjectMolecular Sequence Dataen
dc.subject*Monosaccharide Transport Proteinsen
dc.subjectMutagenesis, Site-Directeden
dc.subjectProtein Structure, Secondaryen
dc.subjectSpecies Specificityen
dc.subjectSpheroplasts/metabolismen
dc.subject*Symportersen
dc.titleBinding of monoclonal antibody 4B1 to homologs of the lactose permease of Escherichia colien
heal.abstractThe conformationally sensitive epitope for monoclonal antibody (mAb) 4B1, which uncouples lactose from H+ translocation in the lactose permease of Escherichia coli, is localized in the periplasmic loop between helices VII and VIII (loop VII/VIII) on one face of a short helical segment (Sun J, et al., 1996, Biochemistry 35;990-998). Comparison of sequences in the region corresponding to loop VII/VIII in members of Cluster 5 of the Major Facilitator Superfamily (MFS), which includes five homologous oligosaccharide/H+ symporters, reveals interesting variations. 4B1 binds to the Citrobacter freundii lactose permease or E. coli raffinose permease with resultant inhibition of transport activity. Because E. coli raffinose permease contains a Pro residue at position 254 rather than Gly, it is unlikely that the mAb recognizes the peptide backbone at this position. Consistently, E. coli lactose permease with Pro in place of Gly254 also binds 4B1. In contrast, 4B1 binding is not observed with either Klebsiella pneumoniae lactose permease or E. coli sucrose permease. When the epitope is transferred from E. coli lactose permease (residues 245-259) to the sucrose permease, the modified protein binds 4B1, but the mAb has no significant effect on sucrose transport. The studies provide further evidence that the 4B1 epitope is restricted to loop VII/VIII, and that 4B1 binding induces a highly specific conformational change that uncouples substrate and H+ translocation.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1002/pro.5560060714-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9232651-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1002/pro.5560060714/asset/5560060714_ftp.pdf?v=1&t=h0byybcx&s=9ad0ef1e81c10a9fe7870771b5e587d33aa67918-
heal.journalNameProtein Scien
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1997-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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