Beta 2-microglobulin and HLA-DR expression in relation to the presence of Epstein-Barr virus in nasopharyngeal carcinomas

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dc.contributor.authorKouvidou, C.en
dc.contributor.authorRontogianni, D.en
dc.contributor.authorTzardi, M.en
dc.contributor.authorDatseris, G.en
dc.contributor.authorPanayiotides, I.en
dc.contributor.authorDarivianaki, K.en
dc.contributor.authorKaridi, E.en
dc.contributor.authorDelides, G.en
dc.contributor.authorKanavaros, P.en
dc.date.accessioned2015-11-24T19:33:10Z
dc.date.available2015-11-24T19:33:10Z
dc.identifier.issn1015-2008-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23505
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectAgeden
dc.subjectAged, 80 and overen
dc.subjectCarcinoma/immunology/virologyen
dc.subjectCarcinoma, Squamous Cell/*immunology/*virologyen
dc.subjectFemaleen
dc.subjectHLA-DR Antigens/*metabolismen
dc.subjectHerpesvirus 4, Human/genetics/*isolation & purificationen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectIn Situ Hybridizationen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectNasopharyngeal Neoplasms/*immunology/*virologyen
dc.subjectRNA, Viral/analysis/geneticsen
dc.subjectRNA-Binding Proteins/geneticsen
dc.subject*Ribosomal Proteinsen
dc.subjectViral Matrix Proteins/metabolismen
dc.subjectbeta 2-Microglobulin/*metabolismen
dc.titleBeta 2-microglobulin and HLA-DR expression in relation to the presence of Epstein-Barr virus in nasopharyngeal carcinomasen
heal.abstractWe have investigated the immunohistochemical expression of beta 2-microglobulin and HLA-DR proteins in nasopharyngeal carcinoma (NPC) in relation to the expression of the Epstein-Barr virus (EBV)-encoded EBER 1-2 mRNAs and LMP-1 protein. beta 2-Microglobulin is expressed in association with MHC-I molecules on most nucleated cells and HLA-DR belongs to the MHC-II molecules which are expressed mostly on antigen-presenting cells. Formalin-fixed paraffin-embedded tissue from 37 NPC cases was stained with immunohistochemistry for beta 2-microglobulin, HLA-DR and LMP-1 proteins and by RNA in situ hybridization for EBER 1-2 mRNAs. beta 2-Microglobulin-positive staining was found in neoplastic cells in all cases. In 19/37 NPC cases the positive staining was found in most neoplastic cells. In the remaining 18 NPC cases more than 25% of the neoplastic cells showed significantly reduced or negative staining in comparison to the normal epithelium and infiltrating small lymphocytes. HLA-DR-positive staining was found in 27/37 NPC cases. EBER 1-2 transcripts were deteced in neoplastic cells in 13/37 NPC cases. LMP-1 expression in tumour cells was found in 4/13 EBER1-2-positive cases. No correlation was found between the presence of EBER1-2 transcripts or LMP-1 protein and the beta 2-microglobulin-reduced expression in NPC. Thus, EBV does not seem to use downregulation of MHC-I to avoid the T cell cytotoxic immune response in NPC. HLA-DR expression was observed in all 13 EBV-positive cases of NPC, suggesting that EBV may participate in the induction of HLA-DR expression in a proportion of NPC.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/8738471-
heal.identifier.secondaryhttp://content.karger.com/ProdukteDB/produkte.asp?doi=10.1159/000163968-
heal.journalNamePathobiologyen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1995-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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