DNA damage and chromosomal aberrations in various types of male factor infertility

Απλή σελίδα τεκμηρίου
dc.contributor.authorPapachristou, F.en
dc.contributor.authorSimopoulou, M.en
dc.contributor.authorTouloupidis, S.en
dc.contributor.authorTsalikidis, C.en
dc.contributor.authorSofikitis, N.en
dc.contributor.authorLialiaris, T.en
dc.date.accessioned2015-11-24T19:24:00Z
dc.date.available2015-11-24T19:24:00Z
dc.identifier.issn1556-5653-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22400
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectCaffeine/toxicityen
dc.subjectCell Shapeen
dc.subjectCell Survivalen
dc.subjectCells, Cultureden
dc.subject*Chromosomal Instabilityen
dc.subject*DNA Damageen
dc.subjectHumansen
dc.subjectInfertility, Male/*genetics/pathologyen
dc.subjectKaryotypingen
dc.subjectLeukocytes/drug effects/pathologyen
dc.subjectMaleen
dc.subjectMitomycin/toxicityen
dc.subjectMutagenicity Testsen
dc.subjectMutagens/toxicityen
dc.subjectProspective Studiesen
dc.subject*Sister Chromatid Exchangeen
dc.subjectSperm Counten
dc.subjectSpermatozoa/pathologyen
dc.subjectYoung Adulten
dc.titleDNA damage and chromosomal aberrations in various types of male factor infertilityen
heal.abstractOBJECTIVE: To understand and delineate the nature and level of DNA damage in association to semen parameters in infertile men. DESIGN: A prospective experimental study. SETTING: Alexandroupolis University General Hospital. PATIENT(S): Eleven fertile and 27 infertile men with various types of infertility. INTERVENTION(S): DNA damage was induced by addition of mitomycin C and caffeine to lymphocyte cultures. MAIN OUTCOME MEASURE(S): Sister chromatid exchange (SCE) levels were assessed in cultures providing a quantitative index of genotoxicity and chromosomal analysis was performed using G-banding and C-banding techniques. RESULT(S): Karyotyping analysis indicated chromosomal fragility, trisomic lines, and marker chromosomes in some infertile men. Double minute chromosomes were noticed in 11 infertile men and were positively correlated with elevated SCE levels. Necrospermia and varicocele, irrespectively of the degree of severity, were positively correlated with elevated SCE levels. CONCLUSION(S): Infertile men are prone to have DNA damage; the nature and level of DNA damage varies and is associated with semen parameters. The presence of double minute chromosomes alone is associated with increased double-stranded breaks and abnormal sperm concentration. This study could provide the basis to establish whether and through which process double minute chromosomes could be related to poor semen parameters and regulation of DNA repair.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1016/j.fertnstert.2007.09.006-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/18082736-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0015028207034760/1-s2.0-S0015028207034760-main.pdf?_tid=f74fa4850c6cc7c958a97c01bfb20a1a&acdnat=1333716559_4020ee924ba7a83c8935d8d0151c34cc-
heal.journalNameFertil Sterilen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2008-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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