Use of ligase chain reaction for enhanced detection of Phytophthora infestans

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dc.contributor.authorTooley, P. W.en
dc.contributor.authorHatziloukas, E.en
dc.contributor.authorScott, D. L.en
dc.contributor.authorCarras, M. M.en
dc.date.accessioned2015-11-24T16:32:50Z
dc.date.available2015-11-24T16:32:50Z
dc.identifier.issn0706-0661-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7593
dc.rightsDefault Licence-
dc.subjectbiotinen
dc.subjectdigoxigeninen
dc.subjectparamagneticen
dc.subjectcolorimetricen
dc.subjectmolecular biologyen
dc.subjectpotato late blighten
dc.subjectribosomal DNAen
dc.subjectreaction assayen
dc.subjectchlamydia-trachomatisen
dc.subjectmitochondrial-DNAen
dc.subjectsequence-analysisen
dc.subjectinfected potatoen
dc.subjectpcr techniqueen
dc.subjectlinkage mapen
dc.subjectidentificationen
dc.titleUse of ligase chain reaction for enhanced detection of Phytophthora infestansen
heal.abstractSequences of the internal transcribed spacer region 2 (ITS 2) of ribosomal DNA from 10 different species of Phytophthora were aligned, and regions of dissimilarity were used to construct ligase chain reaction (LCR) primers for detecting the late blight pathogen, Phytophthora infestans. Ligase chain reaction primers allow differentiation of species down to a single base pair difference. The primers have greater specificity than previously described polymerase chain reaction (PCR) primers and allow differentiation of R infestans, Phytophthora mirabilis, and Phytophthora phaseoli, which contain identical ITS sequences, from many other Phytophthora species. Ligase chain reaction was coupled with PCR to increase sensitivity of P. infestans detection and can serve as an enhancement to existing PCR protocols. By labeling LCR primers with biotin and digoxigenin, the LCR was combined with an ELISA-based detection system to improve sensitivity and ease of application.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondary<Go to ISI>://000178873800007-
heal.journalNameCanadian Journal of Plant Pathology-Revue Canadienne De Phytopathologieen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2002-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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