An Open Reading Frame in the Approximately 28-Kb Tox-Argk Gene-Cluster Encodes a Polypeptide with Homology to Fatty-Acid Desaturases
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Hatziloukas, E.
Panopoulos, N. J.
Delis, S.
Prosen, D. E.
Schaad, N. W.
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peer reviewed
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Part of an apparent open reading frame in the tox-argK gene cluster of Pseudomonas syringae pathovar phaseolicola (Psp) potentially encodes a polypeptide with sequence similarity to fatty acid desaturases (DES). Escherichia coli B expressing this segment under T7 promoter control produced a 34-kDa polypeptide. The possible involvement of a DES in facilitating phaseolotoxin (Ptx) secretion at the low temperatures normally required for its synthesis and the evolutionary implications about the origin of the tox-argK gene cluster are discussed.
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phaseolotoxin, bean halo blight, ornithine carbamoyltransferase, pseudomonas syringae, syringae pv phaseolicola, bacteriophage-t7 rna-polymerase, pseudomonas-syringae, ornithine carbamoyltransferase, copy-number, phaseolotoxin, DNA, expression, cyanobac
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<Go to ISI>://A1995TK18700011
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών