ATR microspectroscopy with multivariate analysis segregates grades of exfoliative cervical cytology
Φόρτωση...
Ημερομηνία
Συγγραφείς
Walsh, M. J.
Singh, M. N.
Pollock, H. M.
Cooper, L. J.
German, M. J.
Stringfellow, H. F.
Fullwood, N. J.
Paraskevaidis, E.
Martin-Hirsch, P. L.
Martin, F. L.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Biochem Biophys Res Commun
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Although cervical cancer screening in the UK has led to reductions in the incidence of invasive disease, this programme remains flawed. We set out to examine the potential of infrared (IR) microspectroscopy to allow the profiling of cellular biochemical constituents associated with disease progression. Attenuated total reflection-Fourier Transform IR (ATR) microspectroscopy was employed to interrogate spectral differences between samples of exfoliative cervical cytology collected into liquid based cytology (LBC). These were histologically characterised as normal (n = 5), low-grade (n = 5), high-grade (n = 5) or severe dyskaryosis (? carcinoma) (n = 5). Examination of resultant spectra was coupled with principal component analysis (PCA) and subsequent linear discriminant analysis (LDA). The interrogation of LBC samples using ATR microspectroscopy with PCA-LDA facilitated the discrimination of different categories of exfoliative cytology and allowed the identification of potential biomarkers of abnormality; these occurred prominently in the IR spectral region 1200 cm(-1) - 950 cm(-1) consisting of carbohydrates, phosphate, and glycogen. Shifts in the centroids of amide I (approximately 1650 cm(-1)) and II (approximately 1530 cm(-1)) absorbance bands, indicating conformational changes to the secondary structure of intracellular proteins and associated with increasing disease progression, were also noted. This work demonstrates the potential of ATR microspectroscopy coupled with multivariate analysis to be an objective alternative to routine cytology.
Περιγραφή
Λέξεις-κλειδιά
Female, Humans, Spectroscopy, Fourier Transform Infrared/*methods, Uterine Cervical Diseases/*pathology
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/17141660
http://ac.els-cdn.com/S0006291X06024594/1-s2.0-S0006291X06024594-main.pdf?_tid=d338c1900ca07e51c435839fc7ee4c58&acdnat=1333714098_49e21f3ec891c978052d6bdae6f66fb6
http://ac.els-cdn.com/S0006291X06024594/1-s2.0-S0006291X06024594-main.pdf?_tid=d338c1900ca07e51c435839fc7ee4c58&acdnat=1333714098_49e21f3ec891c978052d6bdae6f66fb6
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής