A new method for the determination of free L-carnitine in serum samples based on high field single quantum coherence filtering H-1-NMR spectroscopy
| dc.contributor.author | Tsiafoulis, C. G. | en |
| dc.contributor.author | Exarchou, V. | en |
| dc.contributor.author | Tziova, P. P. | en |
| dc.contributor.author | Bairaktari, E. | en |
| dc.contributor.author | Gerothanassis, I. P. | en |
| dc.contributor.author | Troganis, A. N. | en |
| dc.date.accessioned | 2015-11-24T16:56:52Z | |
| dc.date.available | 2015-11-24T16:56:52Z | |
| dc.identifier.issn | 1618-2642 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10501 | |
| dc.rights | Default Licence | - |
| dc.subject | l-carnitine | en |
| dc.subject | serum | en |
| dc.subject | h-1-nmr | en |
| dc.subject | single quantum coherence filtering h-1-nmr | en |
| dc.subject | protein precipitation | en |
| dc.subject | tandem mass-spectrometry | en |
| dc.subject | magnetic-resonance-spectroscopy | en |
| dc.subject | performance liquid-chromatography | en |
| dc.subject | high-resolution nmr | en |
| dc.subject | seminal plasma | en |
| dc.subject | blood-serum | en |
| dc.subject | acylcarnitines | en |
| dc.subject | metabolites | en |
| dc.subject | urine | en |
| dc.subject | quantification | en |
| dc.title | A new method for the determination of free L-carnitine in serum samples based on high field single quantum coherence filtering H-1-NMR spectroscopy | en |
| heal.abstract | The rapid and accurate determination of specific metabolites present in biofluids is a very demanding task which is essential in both medicine and chemistry. l-carnitine (3-hydroxy-4-N-trimethylammonium butyrate) is an important metabolite which participates in a series of biological paths and therefore its determination is of diagnostic importance. A single quantum coherence filtering H-1 NMR methodology was used for the accurate and rapid determination of l-carnitine in human serum samples. The methodology is based on spectral simplification, and specifically on the distinction of the N-methyl proton signal of l-carnitine that is greatly overlapped in the H-1-NMR spectrum of serum. The quantitative results provided by the proposed method are in excellent agreement with those obtained by the enzymatic method, which is widely used. The proposed method is rapid (similar to 20 min of experimental time), selective, sensitive, and has good analytical characteristics (accuracy, reproducibility). Selected protein precipitation methods were also investigated and sample pretreatment with EtOH is suggested. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | DOI 10.1007/s00216-010-4600-0 | - |
| heal.identifier.secondary | <Go to ISI>://000286982900035 | - |
| heal.identifier.secondary | http://download.springer.com/static/pdf/260/art%253A10.1007%252Fs00216-010-4600-0.pdf?auth66=1381649373_a50270a6863e3a1399949181e6748752&ext=.pdf | - |
| heal.journalName | Anal Bioanal Chem | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2011 | - |
| heal.publisher | Springer Verlag (Germany) | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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