Antibodies to Eyrkk Vesicular Stomatitis Virus-Related Peptide Account Only for a Minority of Anti-Ro60kd Antibodies
Φόρτωση...
Ημερομηνία
Συγγραφείς
Routsias, J. G.
Sakarellos-Daitsiotis, M.
Detsikas, E.
Tzioufas, A. G.
Sakarellos, C.
Moutsopoulos, H. M.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Blackwell
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Clin Exp Immunol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Previous studies demonstrated a possible antigenic relation between the carboxyl terminal portion of anti-Ro60kD autoantigen and a nucleocapsid protein (N) of vesicular stomatitis virus (VSV). In order to investigate whether anti-Ro60kD autoantibodies react with the VSV homologous region of the Ro60kD protein we synthesized, according to Merrifield's method, the EYRKKMDI octapeptide (8p) sharing a common sequence with the N protein of VSV. Sera from 61 patients with autoimmune rheumatic diseases (34 systemic lupus erythematosus (SLE), 21 Sjogren's syndrome (SS) and six rheumatoid arthritis (RA)) as well as 59 from normal blood donors were tested for the presence of anti-Ro60kD autoantibodies by ELISA and immunoblot (IB) and anti-8p antibodies by ELISA. Antibodies to 8p were found in 9/31 of anti-Ro60kD IB-positive sera, 5/30 of anti-Ro60kD-negative sera and 2/59 of normal control sera. The concordance between the anti-8p ELISA and the anti-Ro60kD IB was very poor (chi(2) = 0.71, P = 0.4) in contrast to the anti-Ro60kD ELISA and the anti-Ro IB (chi(2) = 27.6, P = 10(-7)). Subsequent affinity purification of the anti-8p antibodies from a strong positive anti-8p and anti-Ro60kD SLE serum yielded 95% depletion of the anti-8p activity and 37% reduction of the anti-Ro60kD activity. Inhibition assays with the affinity-purified anti-8p antibodies demonstrated that the octapeptide gave 94.5% inhibition of the anti-Ro60kD activity, while Ro60kD protein led to 42.3% inhibition of the anti-8p. Preincubation of the serum with the octapeptide produced 4% inhibition of anti-Ro60kD ELISA. These results indicate that the anti-8p antibodies account only for a minority of the anti-Ro60kD autoantibodies.
Περιγραφή
Λέξεις-κλειδιά
vesicular stomatitis virus, autoimmune rheumatic diseases, synthetic peptides, ro/ssa, systemic lupus erythematosus, systemic lupus-erythematosus, ro ribonucleoprotein, sjogrens-syndrome, revised criteria, immunoglobulin-g, proteins, classification, autoantibodies, heterogeneity, epitopes
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://A1994PW37700011
http://onlinelibrary.wiley.com/store/10.1111/j.1365-2249.1994.tb05506.x/asset/j.1365-2249.1994.tb05506.x.pdf?v=1&t=h0e0r6w2&s=dcb8cb726ad742b46f415dff3bfd39ec73e40d4e
http://onlinelibrary.wiley.com/store/10.1111/j.1365-2249.1994.tb05506.x/asset/j.1365-2249.1994.tb05506.x.pdf?v=1&t=h0e0r6w2&s=dcb8cb726ad742b46f415dff3bfd39ec73e40d4e
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας