The destructive action of IL-1alpha and IL-1beta in IDDM is a multistage process: evidence and confirmation by apoptotic studies, induction of intermediates and electron microscopy

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dc.contributor.authorVassiliadis, S.en
dc.contributor.authorDragiotis, V.en
dc.contributor.authorProtopapadakis, E.en
dc.contributor.authorAthanassakis, I.en
dc.contributor.authorMitlianga, P.en
dc.contributor.authorKonidaris, K.en
dc.contributor.authorPapadopoulos, G. K.en
dc.date.accessioned2015-11-24T19:42:09Z
dc.date.available2015-11-24T19:42:09Z
dc.identifier.issn0962-9351-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/24596
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectAntigens, CD95/analysis/biosynthesisen
dc.subjectApoptosis/*drug effectsen
dc.subjectDNA Fragmentationen
dc.subjectDiabetes Mellitus, Type 1/*immunologyen
dc.subjectHumansen
dc.subjectInsulinomaen
dc.subjectInterleukin-1/*toxicityen
dc.subjectKineticsen
dc.subjectMicroscopy, Electronen
dc.subjectMicroscopy, Electron, Scanningen
dc.subjectNecrosisen
dc.subjectNitric Oxide/analysisen
dc.subjectPancreatic Neoplasmsen
dc.subjectRatsen
dc.subjectRecombinant Proteins/toxicityen
dc.subjectTumor Cells, Cultureden
dc.titleThe destructive action of IL-1alpha and IL-1beta in IDDM is a multistage process: evidence and confirmation by apoptotic studies, induction of intermediates and electron microscopyen
heal.abstractUsing the rat beta-cell RIN-5AH insulinoma line as a means for studying insulin-dependent diabetes mellitus (IDDM), it is shown that interleukin-1 (IL-1) induces beta-cell damage initiated by early apoptotic signals. This action is demonstrated by DNA fragmentation, as assessed by specific BrdU labeling, surface expression of Fas and nitric oxide (NO) production. In addition, the interplay between NO and Fas is shown, while scanning electron microscopy (SEM) confirms apoptosis by revealing the degree and type of cellular damage which, in the case of IL-1alpha, can be reversed by an inhibitor to NO synthesis. Apoptosis is also reconfirmed by transmission electron microscopy (TEM) by observing condensed nuclear chromatin after IL-1 exposure. Thus, treatment of insulinoma cells with IL-1alpha and IL-1beta seems to initiate a number of signals, including PKC activation as published previously, that ultimately lead to beta-cell destruction. Each IL-1 isoform, however, definitely follows a different pathway of action.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1080/09629359990577-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/10704145-
heal.identifier.secondaryhttp://downloads.hindawi.com/journals/mi/1999/926797.pdf-
heal.journalNameMediators Inflammen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1999-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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