nu-FBR-fos oncogene fails to rescue mammalian cells from growth arrest but affects the responses of human fibroblasts to heparin
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peer-reviewed
Είδος εκπαιδευτικού υλικού
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Όνομα περιοδικού
Anticancer Res
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Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The effects of nu -fos oncogene on the proliferation of mammalian cells were studied using several approaches. Constitutive overexpression of nu -FBR-fos in normal human fibroblasts (MRC-5) anti of v-FBR-fos in human chondrocytes (HAC21) failed to immortalise them, extend their in vitro lifespan, increase their growth rates or induce cellular transformation. Furhter; nu -FBR-fos did not render- MRC-5 snwth factor independent or alter thier responsivensess to serum but it markedly suppressed their heparin-induced proliferation. A conditionally immortalised, temperature-sensitive rat embryo fibroblast cell line (tsa14) which undergoes growth arrest upon inactivation of a thermolabile SV40 large T antigen by a temperature shift producing a phenotype that mimick the senescent phenotype was also used to study the effects of nu -FBR-fos on cell proliferation. Whereas a wild-type SV40 large T antigen rescued tsa14 from a temperature-dependent growth arrest, v-FBR-fos failed to do so. Hence, v-FBR-fos was not sufficient to, at least, complement the tsa14 growth defect. There was no change in the expression of c-jun and junB, members of the AP-I transcriptional complex in MRC-Sv-fos cells. These darn show that nu -FBR-fos is not sufficient to rescue mammalian cells from senescence but it can affect the responses of human fibroblasts to heparin in suggesting a role of fos in cell proliferation.
Περιγραφή
Λέξεις-κλειδιά
nu-fbr-fos, mammalian cells, cell proliferation, growth arrest, senescence, large-t-antigen, senescent human-fibroblasts, human-diploid fibroblasts, rat embryo fibroblasts, sv40 large t, c-fos, v-fos, cellular senescence, replicative senescence, life-span
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<Go to ISI>://000167875900064
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en
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Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής