Study of the Electrochemical Behavior of Disperse Blue 1-Modified Graphite Electrodes. Application to the Flow Determination of NADH
Φόρτωση...
Ημερομηνία
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
WILEY-VCH Verlag
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Electroanalysis
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The preparation and the electrochemical study of Disperse Blue 1-chemically modified electrodes (DB1-CME), as well as their efficiency for the electrocatalytic oxidation of NADH is described. The proposed mediator was immobilized by physical adsorption onto graphite electrodes. The electrochemical behavior of DB1-CME was studied with cyclic voltammetry. The electrochemical redox reaction of DB1 was found to be reversible, revealing two well-shaped pair of peaks with formal potentials 152 and β�’42"…mV, respectively, (vs. Ag/AgCl/3M KCl) at pH"…6.5. The current Ip has a linear relationship with the scan rate up to 800"…mV"…sβ�’1, which is indicative for a fast electron transfer kinetics. The dissociation constants of the immobilized DB1 redox couple were calculated pK1=4 and pK2=5. The electrochemical rate constants of the immobilized DB1 were calculated k1Β°=18"…sβ�’1 and k2Β°=23"…sβ�’1 (Ξ“=2.36"…nmol"…cmβ�’2). The modified electrodes were mounted in a flow injection manifold, poised at +150"…mV (vs. Ag/AgCl/3M KCl) and a catalytic current due to the oxidation of NADH was measured. The reproducibility was 1.4% RSD (n=11 for 30"…ΞΌM NADH) The behavior of the sensor towards different reducing compounds was investigated. The sensor exhibited good operational and storage stability.
Περιγραφή
Λέξεις-κλειδιά
Disperse Blue 1-modified graphite electrode, Cyclic voltammetry, Electrocatalysis of NADH, Flow injection analysis
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://onlinelibrary.wiley.com/doi/10.1002/elan.200302902/abstract
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας