Targeting c-KIT, PDGFR in cancer of unknown primary: a screening study for molecular markers of benefit
Φόρτωση...
Ημερομηνία
Συγγραφείς
Dova, L.
Pentheroudakis, G.
Golfinopoulos, V.
Malamou-Mitsi, V.
Georgiou, I.
Vartholomatos, G.
Ntemou, A.
Fountzilas, G.
Pavlidis, N.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
J Cancer Res Clin Oncol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
AIMS: In view of available targeted therapies, we investigated the presence of c-kit, PDGFR gene mutations and protein expression in cancer of unknown primary (CUP) in order to study their contribution in pathogenesis, their prognostic value and potential as therapeutic targets. METHODS: Mutations in hot spots c-kit exon 11 and PDGFR exons 12 and 18 were studied in paraffin-embedded tumour samples from 50 patients with CUP by means of PCR-based single-strand conformational polymorphism and protein expression by means of streptavidin-biotin immunoperoxidase assays. Molecular markers were screened for possible correlations with patient outcome. RESULTS: No shifted band was detected in any of the polyacrylamide gel electrophoreses, indicating absence of c-kit exon 11 and PDGFR exon 12, 18 mutations. Immunohistochemical analysis in 37 tumours revealed positive membranous CD117 expression in 30 samples (81%) of which five exhibited strong (+3), four moderate (+2) and 21 weak (+1) staining. PDGFRa protein staining was seen in 15 out of 30 (50%) cases, mostly weak (13) and rarely moderate (1) or strong (1). The expression of KIT or PDGFRa protein did not correlate with the clinical outcome of the patients in our cohort. CONCLUSIONS: In a moderate-sized CUP patient cohort, KIT or PDGFRa protein overexpression is rare, does not have gross prognostic significance for survival and is not associated with presence of activating mutations.
Περιγραφή
Λέξεις-κλειδιά
Aged, Aged, 80 and over, Exons, Female, Gastrointestinal Stromal Tumors/drug therapy/genetics, Humans, Immunohistochemistry, Indoles/therapeutic use, Male, Middle Aged, Mutation, Neoplasms, Unknown Primary/*drug therapy/genetics/mortality, Piperazines/therapeutic use, Polymorphism, Genetic, Prognosis, Proto-Oncogene Proteins c-kit/analysis/*genetics, Pyrimidines/therapeutic use, Pyrroles/therapeutic use, Receptors, Platelet-Derived Growth Factor/analysis/antagonists &, inhibitors/*genetics
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/18064489
http://www.springerlink.com/content/37nkj143gq35w162/fulltext.pdf
http://www.springerlink.com/content/37nkj143gq35w162/fulltext.pdf
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής