Transcriptional analysis of a gene cluster involved in glucose tolerance in Zymomonas mobilis: Evidence for an osmoregulated promoter
Φόρτωση...
Ημερομηνία
Συγγραφείς
Christogianni, A.
Douka, E.
Koukkou, A. I.
Hatziloukas, E.
Drainas, C.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
American Society for Microbiology
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
J Bacteriol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Exponentially growing cells of Zymomonas mobilis normally exhibit a lag period of up to 3 It when they are transferred from a liquid medium containing 2% glucose to a liquid medium containing 10% glucose. A mutant of Z. mobilis (CU1) exhibited a lag period of more than 20 h when it was grown under the same conditions, whereas it failed to grow on a solid medium containing 10% glucose. The glucose-defective phenotype of mutant CU1 was due to a spontaneous insertion in a putative gene (ORF4) identified as part of an operon (glc) which includes three additional putative genes (ORF1, ORF2, and ORF3) with no obvious involvement in the glucose tolerance mechanism. The common promoter controlling glc operon transcription, designated P-glc, was found to be osmoregulated and stimulated by the putative product of OPF4 in an autoregulated fashion, as indicated by expression of the gfp reporter gene. Additionally, reverse transcriptase PCR analysis showed that the gene cluster produces a single mRNA, which verified the operon organization of this transcription unit. Further transcriptional analysis demonstrated that glc operon expression is regulated by the concentration of glucose, which supported the hypothesis that this operon is directly involved in the uncharacterized glucose tolerance mechanism of Z. mobilis.
Περιγραφή
Λέξεις-κλειδιά
green fluorescent protein, rt-pcr, expression, cloning, biosynthesis, pathway, growth, bacteria, isoprenoids, 5-phosphate
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000230716800015
http://jb.asm.org/content/187/15/5179.full.pdf
http://jb.asm.org/content/187/15/5179.full.pdf
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας