Effects of cigarette smoking on the antioxidant defence in young healthy male volunteers
Φόρτωση...
Ημερομηνία
Συγγραφείς
Charalabopoulos, K.
Assimakopoulos, D.
Karkabounas, S.
Danielidis, V.
Kiortsis, D.
Evangelou, A.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Int J Clin Pract
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Cigarette smoking induces a significant oxidant effect related to variety of free radical-related diseases often affecting the upper respiratory tract, unless it is effectively compensated by the antioxidant barriers of the humans. In the present study, the evaluation of the antioxidant compensatory mechanisms, by estimating the antioxidant capacity of extracellular defence (saliva and plasma) and the intracellular resistance of peripheral lymphocytes to oxidative stress in young healthy smokers, was investigated. Twenty young healthy male smokers and 20 age-matched non-smokers with similar dietary profiles were enrolled in the study. Total saliva and plasma samples were collected from both groups, and total antioxidant capacity (TAC) and lag time were estimated. The latter was also repeated in smokers just after a cigarette smoking. Peripheral lymphocytes isolated from the subjects of both groups were also tested for their inherent DNA damage as well as for their ability to resist H2O2-induced DNA damage by using the comet assay. TAC of plasma was found significantly higher in smokers compared to non-smokers (p <0.004), whereas no difference was recorded in plasma lag time values. Lymphocytes of smokers manifested a significantly decreased oxidant resistance (increased DNA fragmentation) to H2O2, in comparison to non-smokers. Our results indicate that young smokers do not manifest different salivary antioxidant defence than non-smokers. They exhibit, however, a higher plasma antioxidant capacity, but a significantly reduced ability of blood lymphocytes, to resist to H2O2-induced DNA damage.
Περιγραφή
Λέξεις-κλειδιά
Adult, Antioxidants/*analysis, Comet Assay/methods, DNA Damage/drug effects, Enzyme-Linked Immunosorbent Assay/methods, Flow Cytometry/methods, Humans, Lymphocytes/blood/drug effects, Male, Oxidative Stress/drug effects, Saliva/chemistry, Smoking/*blood
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/15707460
http://onlinelibrary.wiley.com/store/10.1111/j.1742-1241.2004.00340.x/asset/j.1742-1241.2004.00340.x.pdf?v=1&t=h055453a&s=bc1ddde49baefeaef3ef207aed076f7431195aaa
http://onlinelibrary.wiley.com/store/10.1111/j.1742-1241.2004.00340.x/asset/j.1742-1241.2004.00340.x.pdf?v=1&t=h055453a&s=bc1ddde49baefeaef3ef207aed076f7431195aaa
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής