Equilibrium and structural studies on copper(II) complexes of tetra-, penta- and hexa-peptides containing histidyl residues at the C-termini
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Varnagy, K.
Szabo, J.
Sovago I
Malandrinos, G.
Hadjiliadis, N.
Sanna, D.
Micera, G.
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peer reviewed
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Journal of the Chemical Society-Dalton Transactions
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The stoichiometry, stability constants and solution structure of the complexes formed in the reaction of copper(II) with oligopeptides containing histidyl residues at the C-termini (Gly(3)His, Gly(4)His and Gly(5)His) have been determined by potentiometric, UV-VIS and EPR spectroscopic methods. The formation of the species [CuHL](2+), [CuL](+), [CuH(-1)L], [CuH(-2)L](-) and [CuH(-3)L](2-) was detected in all cases. Binding modes of the species [CuL](+), [CuH(-1)L] and [CuH(-2)L](-) were characterized by the metal ion co-ordination of the terminal amino group, carbonyl oxygen or one or two deprotonated amide nitrogens in joined five-membered chelates from the N-termini, while the fourth co-ordination site of the metal ion was occupied by nitrogen donors of imidazole in the form of a macrochelate. The stability of the macrochelate was decreased upon increasing the length of the peptide molecule. For the penta- and hexa-peptides the species [CuH(-3)L](2-) was characterized as a 4N-complex with equatorial co-ordination of the terminal amino group and subsequent three deprotonated amide nitrogens, with unco-ordinated imidazolyl residues, while a 5N-species was suggested to form for Gly(3)His with axial interaction of the imidazole-N donor atom. Copper(II) complexes of Gly(2)His and pentaglycine were also investigated for reliable comparison.
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nuclear-magnetic-resonance, glycylglycyl-l-histidine, metal-ion complexes, imidazole nitrogens, angiotensin-ii, transport site, serum-albumin, amino, oligopeptides, proton
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<Go to ISI>://000085350300007
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας