Coelomic cells show apoptosis via Fas/FasL system: a comparative study between healthy human pregnancies and missed miscarriages

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dc.contributor.authorKaponis, A.en
dc.contributor.authorSkyrlas, A.en
dc.contributor.authorZagorianakou, N.en
dc.contributor.authorGeorgiou, I.en
dc.contributor.authorPassa, V.en
dc.contributor.authorParaskevaidis, E.en
dc.contributor.authorMakrydimas, G.en
dc.date.accessioned2015-11-24T19:30:06Z
dc.date.available2015-11-24T19:30:06Z
dc.identifier.issn1460-2350-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23021
dc.rightsDefault Licence-
dc.subjectAbortion, Missed/*physiopathologyen
dc.subjectAdulten
dc.subjectAmniotic Fluid/cytologyen
dc.subjectAntigens, CD95/*physiologyen
dc.subjectApoptosis/*physiologyen
dc.subjectFas Ligand Protein/*physiologyen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectIn Situ Nick-End Labelingen
dc.subjectPlacenta/*physiologyen
dc.subjectPregnancyen
dc.subjectPregnancy Trimester, First/*physiologyen
dc.subjectRNA, Messenger/metabolismen
dc.subjectTrophoblasts/cytology/physiologyen
dc.titleCoelomic cells show apoptosis via Fas/FasL system: a comparative study between healthy human pregnancies and missed miscarriagesen
heal.abstractBACKGROUND: The Fas/Fas ligand (FasL) system represents one of the main apoptotic pathways controlling placental apoptosis throughout gestation. In the current study, we have examined the Fas/FasL protein expression and the apoptotic incidents of coelomic cells, amniotic cells and trophoblastic tissue in first trimester human pregnancies and missed miscarriages (MM). METHODS: Protein expression was determined by immunofluoresence, western blotting analysis, immunohistochemistry and indirectly by RT-PCR, whereas apoptotic cell death was assessed by in situ DNA fragmentation analysis. RESULTS: Coelomic cells express Fas/FasL proteins, can undergo apoptosis and were the only cells in which apoptosis, Fas protein expression and FasL protein expression were accordingly increased along with gestational age (P = 0.001, P = 0.008; P = 0.012, respectively). In contrast, amniotic cells and trophoblast showed a consistency in the expression levels of Fas/FasL proteins in healthy pregnancies. MM were accompanied by increased Fas/FasL protein expression in all examined samples (P < 0.001). The increase of Fas/FasL protein expression was accompanied by proportional increase of apoptotic incidents among the coelomic cell population (P = 0.023, P = 0.009, respectively), whereas amniotic cells and trophoblast appeared to be resistant to Fas-induced apoptosis. The lowest expression of Fas/FasL proteins and the minimum occurrence of apoptotic incidents were detected in the trophoblast. CONCLUSIONS: These data suggest that there is a different regulation and function of the Fas/FasL system in early human pregnancies. Aberration of the Fas-mediated apoptosis may represent one of the execution-step necessary for pregnancy loss in MM cases.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1093/humrep/den031-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/18316328-
heal.identifier.secondaryhttp://humrep.oxfordjournals.org/content/23/5/1159.full.pdf-
heal.journalNameHum Reproden
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2008-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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