Development of an amperometric biosensing method for the determination of L-fucose in pretreated urine
Φόρτωση...
Ημερομηνία
Συγγραφείς
Tsiafoulis, C. G.
Prodromidis, M. I.
Karayannis, M. I.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Biosens Bioelectron
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The first amperometric biosensing method for the determination of L-fucose is described. L-Fucose is the objective of much current research, as it is considered as a potential marker for various pathologic disorders. Recombinant L-fucose dehydrogenase, having as cofactor beta-nicotinamide adenine dinucleotide phosphate (NAD(+)P), was cross-linked in a water-soluble photosensitive polymer matrix, that is, polyvinyl alcohol (PVA) modified with styrylpyridinium (SbQ), in the presence of BSA and glutaraldehyde. The resulting membrane was sandwiched between two polycarbonate membranes and was mounted in an amperometric cell. The oxidation of the enzymatically produced NADPH was monitored at a platinum anode at +0.25 V versus a silver pseudoreference electrode in the presence of ferricyanide. The system was fully optimized with respect to various analytical parameters. Regarding to the mechanical properties of the membrane and the storage stability of the immobilized enzyme, various parameters were also optimized. Several methods for the pretreatment of urine samples were investigated. Treatment of the samples with PbO2 found to eliminate the interference effect of various electroactive species exist in urine; optimum incubation time was determined since at prolonged incubation times L-fucose is also affected. Calibration curves for the direct and the mediated monitoring of NADPH were liner over the concentration ranges 0.04-1.0 mM (r(2) = 0.9995) and 0.03-3.0 mM (r(2) = 0.9997) fucose, respectively. The detection limits (S/N 3) were 2 and 1.5 muM fucose, respectively. The R.S.D. of the mediated biosensor is better than 1.5% (n = 10, 0.5 mM fucose). The proposed biosensor correlates well with a reference enzymatic method and exhibits very good working and storage stability. (C) 2004 Elsevier B.V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
fucose biosensing method, pretreated urine, polyvinyl alcohol-styrylpyridinium, fucose dehydrogenase, lead dioxide, ferricyanide, performance liquid-chromatography, alpha-l-fucose, acid, glucose, serum, assay, electrophoresis, glycoproteins, derivatives, pyruvate
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000225009000030
http://ac.els-cdn.com/S0956566304001393/1-s2.0-S0956566304001393-main.pdf?_tid=a56a93e3f78dcd234fb4748014f993fe&acdnat=1333037431_0cc005ff4c31b387cfff58686017cbd9
http://ac.els-cdn.com/S0956566304001393/1-s2.0-S0956566304001393-main.pdf?_tid=a56a93e3f78dcd234fb4748014f993fe&acdnat=1333037431_0cc005ff4c31b387cfff58686017cbd9
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας