Allelic imbalance of expression and epigenetic regulation within the alpha-synuclein wild-type and p.Ala53Thr alleles in Parkinson disease

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dc.contributor.authorVoutsinas, G. E.en
dc.contributor.authorStavrou, E. F.en
dc.contributor.authorKarousos, G.en
dc.contributor.authorDasoula, A.en
dc.contributor.authorPapachatzopoulou, A.en
dc.contributor.authorSyrrou, M.en
dc.contributor.authorVerkerk, A. J.en
dc.contributor.authorvan der Spek, P.en
dc.contributor.authorPatrinos, G. P.en
dc.contributor.authorStoger, R.en
dc.contributor.authorAthanassiadou, A.en
dc.date.accessioned2015-11-24T19:37:05Z
dc.date.available2015-11-24T19:37:05Z
dc.identifier.issn1098-1004-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23985
dc.rightsDefault Licence-
dc.subjectAllelesen
dc.subject*Allelic Imbalanceen
dc.subjectAmino Acid Substitutionen
dc.subjectCell Line, Transformeden
dc.subjectEpigenesis, Geneticen
dc.subjectFemaleen
dc.subjectGene Dosageen
dc.subjectGene Expressionen
dc.subjectHistones/metabolismen
dc.subjectHumansen
dc.subject*Mutationen
dc.subjectParkinson Disease/*genetics/metabolismen
dc.subjectPolymorphism, Single Nucleotideen
dc.subjectProtein Processing, Post-Translationalen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectalpha-Synuclein/*geneticsen
dc.titleAllelic imbalance of expression and epigenetic regulation within the alpha-synuclein wild-type and p.Ala53Thr alleles in Parkinson diseaseen
heal.abstractGenetic alterations in the alpha-synuclein (SNCA) gene have been implicated in Parkinson Disease (PD), including point mutations, gene multiplications, and sequence variations within the promoter. Such alterations may be involved in pathology through structural changes or overexpression of the protein leading to protein aggregation, as well as through impaired gene expression. It is, therefore, of importance to specify the parameters that regulate SNCA expression in its normal and mutated state. We studied the expression of SNCA alleles in a lymphoblastoid cell line and in the blood cells of a patient heterozygous for p.Ala53Thr, the first mutation to be implicated in PD pathogenesis. Here, we provide evidence that: (1) SNCA shows monoallelic expression in this patient, (2) epigenetic silencing of the mutated allele involves histone modifications but not DNA methylation, and (3) steady-state mRNA levels deriving from the normal SNCA allele in this patient exceed those of the two normal SNCA alleles combined, in matching, control individuals. An imbalanced SNCA expression in this patient is thus documented, with silencing of the p.Ala53Thr allele and upregulation of the wild-type-allele. This phenomenon is demonstrated for a first time in the SNCA gene, and may have important implications for PD pathogenesis.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1002/humu.21248-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/20340137-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1002/humu.21248/asset/21248_ftp.pdf?v=1&t=h0dic38x&s=0288d753d3b7355136c020b5bd72287cb11d4f1a-
heal.journalNameHum Mutaten
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2010-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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