Mechanism of action of prothymosin alpha in the human autologous mixed lymphocyte response

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dc.contributor.authorBaxevanis, C. N.en
dc.contributor.authorReclos, G. J.en
dc.contributor.authorEconomou, M.en
dc.contributor.authorArsenis, P.en
dc.contributor.authorKatsiyiannis, A.en
dc.contributor.authorSeferiades, K.en
dc.contributor.authorPapadopoulos, G.en
dc.contributor.authorTsolas, O.en
dc.contributor.authorPapamichail, M.en
dc.date.accessioned2015-11-24T19:05:06Z
dc.date.available2015-11-24T19:05:06Z
dc.identifier.issn0892-3973-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/20145
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectDose-Response Relationship, Immunologicen
dc.subjectHumansen
dc.subjectInterleukin-1/analysisen
dc.subjectInterleukin-2/analysisen
dc.subject*Lymphocyte Culture Test, Mixeden
dc.subjectMonocytes/drug effects/immunologyen
dc.subjectProtein Precursors/*pharmacologyen
dc.subjectT-Lymphocytes/drug effects/immunologyen
dc.subjectThymosin/*analogs & derivatives/pharmacologyen
dc.subjectTime Factorsen
dc.titleMechanism of action of prothymosin alpha in the human autologous mixed lymphocyte responseen
heal.abstractProthymosin alpha(Prot alpha), an immunologically active polypeptide derived initially from rat thymus, and now pig thymus, was tested for its effect on autoantigen-induced human T cell proliferation in vitro. Pig ProT alpha was found to enhance the autologous mixed lymphocyte response (auto-MLR). Optimum enhancement was achieved at doses which varied among different donors. Treatment of the stimulatory monocytes with ProT alpha resulted in considerably higher auto-MLR responses as compared to those with non treated monocytes. ProT alpha was without effect on T lymphocytes. In contrast, T lymphocytes exhibited enhanced proliferative activity when treated with ProT alpha in the environment of autologous monocytes. Moreover, supernatants from cultures of monocytes incubated with ProT alpha (ProT alpha-sup) were also shown to enhance the human auto-MLR either after addition in cultures or after preincubation with responder T lymphocytes. In addition, ProT alpha-sup did not demonstrate any detectable interleukin 1 (IL 1) or interleukin 2 (IL 2) - like activity. Furthermore, ProT alpha-sup induced an increase in IL 2 production in auto-MLR cultures. The enhancement of T-cell proliferation and IL 2 production by ProT alpha-sup was maximal when this material was added at the beginning of the auto-MLR, and no effect of ProT alpha-sup was seen if the latter was added 3 days after initiation of the culture. Finally, Prot alpha-sup was also shown to increase the expression of IL 2 receptors on T lymphocytes activated in the auto-MLR. These studies suggest that ProT alpha enhances the human auto-MLR through ProT alpha-sup which is released after interaction of monocytes with ProT alpha ProT alpha-sup then increases directly T lymphocyte proliferation by elevating IL 2 production and expression of IL 2 specific receptors on autoactivated T lymphocytes.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.3109/08923978809006448-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/2977606-
heal.identifier.secondaryhttp://informahealthcare.com/doi/abs/10.3109/08923978809006448-
heal.journalNameImmunopharmacol Immunotoxicolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1988-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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