EEA1 links PI(3)K function to Rab5 regulation of endosome fusion

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dc.contributor.authorSimonsen, A.en
dc.contributor.authorLippe, R.en
dc.contributor.authorChristoforidis, S.en
dc.contributor.authorGaullier, J. M.en
dc.contributor.authorBrech, A.en
dc.contributor.authorCallaghan, J.en
dc.contributor.authorToh, B. H.en
dc.contributor.authorMurphy, C.en
dc.contributor.authorZerial, M.en
dc.contributor.authorStenmark, H.en
dc.date.accessioned2015-11-24T19:23:09Z
dc.date.available2015-11-24T19:23:09Z
dc.identifier.issn0028-0836-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22258
dc.rightsDefault Licence-
dc.subjectAndrostadienes/pharmacologyen
dc.subjectAnimalsen
dc.subjectAutoantigens/physiologyen
dc.subjectCattleen
dc.subjectCell Lineen
dc.subjectCloning, Molecularen
dc.subjectCricetinaeen
dc.subjectEndosomes/*physiologyen
dc.subjectEnzyme Inhibitors/pharmacologyen
dc.subjectGTP-Binding Proteins/genetics/*physiologyen
dc.subjectGuanosine Triphosphate/physiologyen
dc.subjectHeLa Cellsen
dc.subjectHumansen
dc.subjectIntracellular Membranes/physiologyen
dc.subjectMembrane Fusion/*physiologyen
dc.subjectMembrane Proteins/genetics/*physiologyen
dc.subjectMutagenesisen
dc.subjectPhosphatidylinositol 3-Kinases/antagonists & inhibitors/*physiologyen
dc.subjectRecombinant Fusion Proteins/metabolismen
dc.subjectVesicular Transport Proteinsen
dc.subjectrab5 GTP-Binding Proteinsen
dc.titleEEA1 links PI(3)K function to Rab5 regulation of endosome fusionen
heal.abstractGTPases and lipid kinases regulate membrane traffic along the endocytic pathway by mechanisms that are not completely understood. Fusion between early endosomes requires phosphatidylinositol-3-OH kinase (PI(3)K) activity as well as the small GTPase Rab5. Excess Rab5-GTP complex restores endosome fusion when PI(3)K is inhibited. Here we identify the early-endosomal autoantigen EEA1 which binds the PI(3)K product phosphatidylinositol-3-phosphate, as a new Rab5 effector that is required for endosome fusion. The association of EEA1 with the endosomal membrane requires Rab5-GTP and PI(3)K activity, and excess Rab5-GTP stabilizes the membrane association of EEA1 even when PI(3)K is inhibited. The identification of EEA1 as a direct Rab5 effector provides a molecular link between PI(3)K and Rab5, and its restricted distribution to early endosomes indicates that EEA1 may confer directionality to Rab5-dependent endocytic transport.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1038/28879-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9697774-
heal.journalNameNatureen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1998-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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