The polyadenylation inhibitor cordycepin (3'dA) causes a decline in c-MYC mRNA levels without affecting c-MYC protein levels

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dc.contributor.authorIoannidis, P.en
dc.contributor.authorCourtis, N.en
dc.contributor.authorHavredaki, M.en
dc.contributor.authorMichailakis, E.en
dc.contributor.authorTsiapalis, C. M.en
dc.contributor.authorTrangas, T.en
dc.date.accessioned2015-11-24T16:32:12Z
dc.date.available2015-11-24T16:32:12Z
dc.identifier.issn0950-9232-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7526
dc.rightsDefault Licence-
dc.subjectDeoxyadenosines/*pharmacologyen
dc.subjectGene Expression Regulation, Neoplastic/drug effectsen
dc.subjectHeLa Cellsen
dc.subjectHumansen
dc.subjectMutagens/*pharmacologyen
dc.subjectPoly A/metabolismen
dc.subjectProtein Biosynthesisen
dc.subjectProto-Oncogene Proteins c-myc/genetics/*metabolismen
dc.subjectRNA, Messenger/*metabolismen
dc.titleThe polyadenylation inhibitor cordycepin (3'dA) causes a decline in c-MYC mRNA levels without affecting c-MYC protein levelsen
heal.abstractStudy of the distribution of the poly(A) tail length of c-myc mRNA in several cell lines revealed a distinct, prevailing population with short poly(A) tails, derived through sequential deadenylation. To elucidate the possible in vivo function of this distinct short tailed c-myc mRNA population, the polyadenylation inhibitor cordycepin was used. This resulted in a decline in steady state c-myc mRNA levels with the remaining messenger mostly oligoadenylated. However, c-MYC proteins did not follow the reduction of the c-myc mRNA. On the other hand, in cells exposed to physiological agents known to downregulate c-myc expression, the reduction of mRNA steady state levels, was reflected upon c-MYC protein levels. The dissociation between c-myc mRNA and protein levels caused by cordycepin was not due to the stabilization of the c-MYC proteins and was not an indiscriminate effect since in the presence of cordycepin, c-fos mRNA and protein levels concomitantly declined. Our data indicate that under these conditions, a long poly(A) tail is not instrumental for c-myc mRNA translation and furthermore, the discrepancy in the steady state of c-myc mRNA level: c-MYC protein ratio between control cells and cells treated with cordycepin indicates that c-myc mRNA is subjected to translational control.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1038/sj.onc.1202255-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9926926-
heal.identifier.secondaryhttp://www.nature.com/onc/journal/v18/n1/pdf/1202255a.pdf-
heal.journalNameOncogeneen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate1999-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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