Sensitivity enhancement of liquid chromatographic-direct chemiluminescence detection by on-line post-column solvent mediated pre-oxidative chemiluminescence
Φόρτωση...
Ημερομηνία
Συγγραφείς
Tsogas, G. Z.
Giokas, D. L.
Vlessidis, A. G.
Evmiridis, N. P.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Chromatography A
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The compatibility of liquid chromatography solvents with oxidizing reagents frequently employed in direct chemiluminescence reactions is examined in this study. Various oxidizing reagents were examined for their response in hydro-organic and micellar mobile phases in both isocratic and gradient elution modes. Mild oxidants like hydrogen peroxide, periodate, cerium and hypochlorite were found to be completely compatible with common reversed phase HPLC solvents posing as no threat to the detection procedure. On the other hand, stronger oxidants like acidic permanganate were found to oxidize organic solvents towards the production of an intense light signal. Although several analytical applications can emerge from this finding, the conjunction of this system with reversed phase HPLC is impractical owing to a significant baseline increase which deteriorates the sensitivity of the analysis. A convenient solution to this problem is proposed based on the regulated on-line post-column pre-oxidation of the organic solvent (SPOC) with mild oxidants that have no influence on the final signal. The analytical utility of this new approach in the determination of organic compounds after chromatographic separation is demonstrated. (c) 2005 Elsevier B.V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
liquid chromatography, direct chemiluminescence, solvent pre-oxidation, acidic potassium-permanganate, electrogenerated chemiluminescence, flow-injection, electrophoresis
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000235505100026
http://ac.els-cdn.com/S002196730600046X/1-s2.0-S002196730600046X-main.pdf?_tid=35c8dc54-356b-11e3-9b2a-00000aab0f26&acdnat=1381822213_c264ede4536d061b4f06ada7b1eab900
http://ac.els-cdn.com/S002196730600046X/1-s2.0-S002196730600046X-main.pdf?_tid=35c8dc54-356b-11e3-9b2a-00000aab0f26&acdnat=1381822213_c264ede4536d061b4f06ada7b1eab900
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας